Abstract
The mitogen-activated protein (MAP) kinases (p44mapk and p42mapk), also known as extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), are activated in response to a variety of extracellular signals, including growth factors, hormones and, neurotransmitters. We have investigated MAP kinase signal transduction pathways in normal human osteoblastic cells. Normal human bone marrow stromal (HBMS), osteoblastic (HOB), and human (TE85, MG-63, SaOS-2), rat (ROS 17/2.8, UMR-106) and mouse (MC3T3-E1) osteoblastic cell lines contained immunodetectable p44mapk/ERK1 and p42mapk/ERK2. MAP kinase activity was measured by 'in-gel' assay using myelin basic protein as the substrate. Mainly ERK2 was rapidly activated (within 10 min) by bFGF, IGF-I and PDGF-BB in normal HOB, HBMS and human osteosarcoma cells, whereas both ERK1 and ERK2 were activated by growth factors in rat osteoblast-like cell lines, ROS 17/2.8 and UMR-106. The ERK1 activation was greater than the ERK2 in ROS 17/2.8 cells. Furthermore, ERK2 was also activated by bFGF and PDGF-BB in the mouse osteoblastic cell line, MC3T3-E1. This is the first demonstration of inter-species differences in the activation of MAP kinases in osteoblastic cells. Cyclic AMP derivatives or cA...Continue Reading
Citations
Aug 8, 2001·European Journal of Haematology·B FossO Bruserud
Oct 28, 1999·Endocrinology·M MiwaT Uematsu
Feb 28, 2002·European Journal of Haematology·B FossØ Bruserud
Jan 1, 2014·International Journal of Molecular Medicine·Jun YokotaAkira Ishisaki
Apr 14, 2017·Journal of Cardiovascular Pharmacology and Therapeutics·Tanuka DattaNabanita S Datta
May 24, 2001·Journal of Biomedical Materials Research·Z SchwartzB D Boyan
Sep 8, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A M MartelliL M Neri
May 7, 2004·The Journal of Biological Chemistry·Chen ChenLaurie K McCauley
Apr 1, 2000·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·M Garcia-RamirezL Audi
Aug 17, 1999·The Journal of Clinical Investigation·J M Hock
May 1, 2001·Journal of Cellular Biochemistry·N GabarinI Bab
Oct 18, 2000·Journal of Biomedical Materials Research·E A CowlesG A Gronowicz
Jan 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sunil WadhwaCarol C Pilbeam
Feb 1, 2002·Journal of Dental Research·S J WebbS Koka
Apr 2, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ming Zhao ChengLance E Lanyon
Apr 17, 2002·Endocrinology·Teresa A DoggettNicola C Partridge
Oct 31, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shilpa ChoudharyCarol C Pilbeam
Sep 26, 2006·Canadian Journal of Physiology and Pharmacology·D A Barrio, S B Etcheverry
Jan 18, 2005·Cell and Tissue Research·Maria Giovanna SabbietiMarja M Hurley
Nov 10, 2005·Blood·Guido BispingJoachim Kienast
Nov 17, 2006·American Journal of Physiology. Renal Physiology·W Bruce SneddonPeter A Friedman
Mar 29, 2000·The Journal of Biological Chemistry·R K JaiswalM F Pittenger
Oct 30, 2004·The Journal of Biological Chemistry·Ling QinNicola C Partridge
Jul 25, 2014·Oral Diseases·F AhmadV Manganiello
Dec 22, 2004·The Journal of Biological Chemistry·Colin A SymeAlessandro Bisello
Mar 13, 2021·European Journal of Pharmacology·Konica PorwalNaibedya Chattopadhyay
Aug 3, 2001·Blood Cells, Molecules & Diseases·M W Long
Jun 14, 2002·Biochimica Et Biophysica Acta·Enrique RosadoBarbara D Boyan
Feb 12, 2000·Prostaglandins, Leukotrienes, and Essential Fatty Acids·L C CarpioR Dziak
Jul 6, 2000·Prostaglandins, Leukotrienes, and Essential Fatty Acids·L C CarpioR Dziak
Sep 11, 2002·Biochemical and Biophysical Research Communications·Sunil WadhwaCarol Pilbeam
May 24, 2003·Biochemical and Biophysical Research Communications·Edelgard Kaiser, Srinivasan Chandrasekhar
Jan 20, 2004·Seminars in Nephrology·Keith A HruskaKameswaran Surendran
Dec 13, 2005·Kidney International. Supplement·Keith A HruskaRichard J Lund
Aug 10, 2010·Biomacromolecules·Mara L MacdonaldPaula T Hammond
May 20, 2008·Bone·Lingjie FuKerong Dai