Identification and analysis of a class 2 alpha-mannosidase from Aspergillus nidulans

Glycobiology
C J EadesW Hintz

Abstract

A Class 2 alpha-mannosidase gene was cloned and sequenced from the filamentous fungus Aspergillus nidulans. A portion of the gene was amplified using degenerate oligonucleotide primers which were designed based on similarity between the Saccharomyces cerevisiae vacuolar and rat ER/cytosolic Class 2 protein sequences. The PCR amplification product was used to isolate the full length gene, and DNA sequencing revealed a 3383 bp coding region containing three introns. The predicted 1049 amino acid reading frame contained six potential N-glycosylation sites and encoded a protein of 118 kDa. The protein sequence did not appear to encode a typical fungal signal sequence or membrane spanning domain. Although the cellular location of the A.nidulans mannosidase was not determined, experimental evidence suggested that it was located within a subcellular organelle. The Matchbox sequence similarity matrix indicated that the A.nidulans protein sequence was more highly similar to the rat ER/cytosolic (Rij = 0.33) and S.cerevisiae vacuolar alpha-mannosidases (Rij = 0.43) than the rat and yeast sequences were to each other (Rij = 0.29). These three enzymes were found to be distantly related to other Class 2 sequences, and compose a third subgro...Continue Reading

References

Oct 1, 1992·Computer Applications in the Biosciences : CABIOS·E Depiereux, E Feytmans
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S Henikoff, J G Henikoff
May 11, 1992·Nucleic Acids Research·J L Cenis
Sep 15, 1989·Biochemical and Biophysical Research Communications·T Yoshihisa, Y Anraku
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K W Moremen
Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Oct 1, 1994·Glycobiology·P F DanielC D Warren
Apr 1, 1994·Glycobiology·K W MoremenA Herscovics
Apr 15, 1994·Biochemical and Biophysical Research Communications·V L Nebes, M C Schmidt
Oct 15, 1993·European Journal of Biochemistry·E BauseB Schmidt
Nov 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G OpdenakkerR A Dwek
Apr 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Herscovics, P Orlean
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·M MisagoM N Fukuda
Jun 1, 1996·The Biochemical Journal·B Henrissat, A Bairoch

❮ Previous
Next ❯

Citations

Nov 29, 2002·Eukaryotic Cell·Fulvio Reggiori, Daniel J Klionsky
Mar 11, 2000·Molecular Biology and Evolution·D S Gonzalez, I K Jordan
Dec 10, 2015·Environmental Monitoring and Assessment·A H YellickM L Otte
Sep 28, 2020·FEBS Open Bio·Jianxiu ZhangKeqiong Ye

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.