Identification and analysis of an Autographa californica nuclear polyhedrosis virus structural protein of the occlusion-derived virus envelope: ODV-E56

Virology
S C BraunagelM D Summers

Abstract

An Autographa californica nuclear polyhedrosis virus gene encoding an occlusion-derived virus (ODV) envelope protein of 56 kDa was identified and sequenced. Transcription initiates from a conserved baculovirus late motif (ATAAG) with transcripts detected from 16 through 72 hr p.i. The protein is detected in infected cell extracts from 36 hr p.i. Western blot assay of ODV, BV, viral envelope, and nucleocapsid preparations coupled with immunoelectron microscopy reveal that this protein localizes to the ODV envelope. This protein is named ODV-E56 to identify its viral origin, envelope location, and apparent molecular weight. ODV-E56 is enriched in viral induced intranuclear microvesicles as determined by immunogold labeling. A mutant was constructed with the C-terminal portion of the protein replaced with beta-galactosidase. The fusion protein, E56-beta-gal, locates to the viral nucleocapsids and not to the ODV envelope or intranuclear microvesicles. This suggests that the signals necessary for transport and/or retention into these structures lies within the C-terminal portion of ODV-E56. Additionally, both ODV-E56 and E56-beta-gal are enriched in electron dense regions that cluster around the inner nuclear membrane and within the...Continue Reading

Citations

May 7, 2010·Journal of Virology·Ranran WangZhihong Hu
May 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sharon C BraunagelMax D Summers
Aug 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·S C BraunagelM D Summers
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sharon C BraunagelMax D Summers
Jan 1, 1996·Cytotechnology·G W Blissard
Nov 5, 2002·Annual Review of Entomology·Elisabeth A HerniouDavid R O'Reilly
Jun 13, 2009·Molekuliarnaia genetika, mikrobiologiia i virusologiia·I M Kikhno
May 23, 2009·Journal of Invertebrate Pathology·Annie BézierJean-Michel Drezen
Jul 1, 2011·International Journal of Evolutionary Biology·Solange Ana Belen MielePablo Daniel Ghiringhelli
May 23, 2006·Nature Structural & Molecular Biology·Suraj SaksenaSharon C Braunagel
Oct 22, 2008·The Journal of General Virology·Qing ZhouFeng Yang
Jan 21, 2014·The Journal of General Virology·Carla Torres BraconiPaolo Marinho de Andrade Zanotto
Oct 11, 2003·Journal of Biochemistry and Molecular Biology·Kianoush Khajeh RashidanClaude Guertin
Jul 10, 1998·Virology·G R Whittaker, A Helenius
Sep 22, 2000·Virology·Y HashimotoT Matsumoto
Jun 12, 2012·Virology·Virginia L DickisonDavid A Theilmann
Apr 27, 2011·Journal of Proteome Research·Xiao-Feng WangChuan-Xi Zhang
Jul 19, 2018·Insects·Bob BoogaardJan W M van Lent
Jul 28, 2018·Molecular Biology and Evolution·Gaelen R BurkeScott M Geib

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