Identification and analysis of an interleukin 8-like molecule in rainbow trout Oncorhynchus mykiss

Developmental and Comparative Immunology
K J LaingChristopher J Secombes

Abstract

An interleukin 8 (IL-8) homologue has been identified in the rainbow trout Oncorhynchus mykiss. The transcript contains an open reading frame of 294 nucleotides that translates into a 97 amino acid putative peptide, with 5' and 3' untranslated regions (UTR) of 171 and 453 nucleotides, respectively. As with previously sequenced lamprey and flounder genes, the trout amino acid sequence lacks the typical ELR motif upstream of the first pair of cysteines, where DLR is present. The trout IL-8 gene contains four exons divided by three short introns of 341, 247 and 292bp, and occupies 1824bp of genomic DNA. RT-PCR reveals a low level constitutive expression of the IL-8 homologue in many tissues, including spleen, heart, liver, head kidney and gill. Expression was not detectable in the brain. Whilst no apparent affect of lipopolysaccharide (LPS) on IL-8 expression was observed in vivo, stimulation of a trout macrophage cell line (RTS-11) with either LPS or poly I:C did result in clear up-regulation of IL-8 expression, detectable by RT-PCR and Northern blot analysis.

References

Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D CaputA Cerami
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Dec 16, 1987·Biochemical and Biophysical Research Communications·A WalzM Baggiolini
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T YoshimuraE J Leonard
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Mar 1, 1970·Journal of Molecular Biology·S B Needleman, C D Wunsch
Aug 13, 1993·Cell·A B Sachs
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·M Hartl, K Bister
Apr 1, 1996·Microbial Pathogenesis·M A MorseyL A Babiuk
Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Jan 23, 1999·Immunological Reviews·B DixonP Parham
Mar 8, 2000·Cytokine·C SickK C Weining

❮ Previous
Next ❯

Citations

Jun 26, 2013·Archives of Environmental Contamination and Toxicology·Huiying LiQian Xiong
Dec 3, 2008·Marine Biotechnology·Zhonghua CaiKezhi Xing
Apr 6, 2004·Developmental and Comparative Immunology·Pete KaiserSucharitha Balu
Apr 6, 2004·Developmental and Comparative Immunology·Kerry J Laing, Christopher J Secombes
Apr 6, 2004·Developmental and Comparative Immunology·Giuseppe ScapigliatiChristopher J Secombes
Mar 27, 2004·Developmental and Comparative Immunology·Puttharat BaoprasertkulZhanjiang Liu
May 25, 2013·Developmental and Comparative Immunology·Jun ChenJun Zou
Jun 14, 2005·Molecular Immunology·Puttharat BaoprasertkulZhanjiang Liu
Oct 17, 2001·Developmental and Comparative Immunology·C J SecombesJ Zou
Jun 24, 2003·Developmental and Comparative Immunology·J ZouC J Secombes
Jul 26, 2003·Developmental and Comparative Immunology·Mark O HuisingB M Lidy Verburg-van Kemenade
Apr 12, 2002·Veterinary Immunology and Immunopathology·Scott PeddieChristopher J Secombes
Aug 2, 2003·Fish & Shellfish Immunology·Yuuki InoueTeruyuki Nakanishi
Aug 29, 2013·Developmental and Comparative Immunology·Bertrand Collet
Aug 7, 2013·Developmental and Comparative Immunology·Young-Mao ChenTzong-Yueh Chen
Nov 17, 2015·Biology·Steve Bird, Carolina Tafalla
Oct 3, 2012·Fish & Shellfish Immunology·Eve MartinJean-Pol Frippiat
Apr 19, 2011·Fish & Shellfish Immunology·Jin-sheng SunLi Sun
May 3, 2011·Developmental and Comparative Immunology·Xianwei CuiShuangquan Zhang
Mar 19, 2011·Developmental and Comparative Immunology·Alí Alejo, Carolina Tafalla
Aug 25, 2009·Fish & Shellfish Immunology·Nevien K AbdelkhalekMiki Nakao
Jun 24, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Sang Jung AhnHyung Ho Lee
Oct 31, 2008·Veterinary Immunology and Immunopathology·Xiang Wan, Xinhua Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.