Identification and analysis of fipA, a Fusobacterium nucleatum immunosuppressive factor gene.

Infection and Immunity
D R DemuthB J Shenker

Abstract

We have previously demonstrated that sonic extracts of Fusobacterium nucleatum FDC 364 were capable of inhibiting human T-cell responses to mitogens and antigens. The purified F. nucleatum immunosuppressive protein (FIP) is composed of two subunits of 44 and 48 kDa. Furthermore, FIP inhibits T-cell activation by arresting cells in the middle of the G(1) phase of the cell cycle; the data available to date suggest that FIP impairs the expression of the proliferating-cell nuclear antigen. To initiate delineation of FIP structure-function relationships, molecular cloning of the FIP gene was carried out. A DNA library of F. nucleatum FDC 364 was constructed by partial digestion of genomic DNA with Sau3A and screened for the production of FIP with polyclonal antibody. Twelve immunoreactive clones were identified. One of these clones contained a 3.1-kbp insert and was chosen for further study. Cell lysates were found to contain an immunoreactive band that comigrated with the 44-kDa subcomponent of the native FIP. Sequencing of the 3.1-kpb insert revealed the presence of three open reading frames (ORFs). One ORF extends from nucleotides 415 to 1620, encodes 402 amino acids, and is preceded by a ribosome-binding site. Deletion analysis ...Continue Reading

References

Feb 1, 1976·Journal of Periodontal Research·B L WilliamsJ C Sherris
May 11, 1975·Journal of Endodontics·W C Wittgow, C B Sabiston
Jun 1, 1975·Bacteriological Reviews·J H Schwab
Jan 1, 1992·Immunopharmacology and Immunotoxicology·B J ShenkerI M Shapiro
Feb 28, 1989·Biochemical and Biophysical Research Communications·E T LallyC W Gibson
Sep 1, 1988·Journal of Periodontal Research·K YamashitaK Hara
Jan 1, 1986·Journal of Cellular Biochemistry·D EisenbergA D McLachlan
Apr 29, 1985·Biochimica Et Biophysica Acta·L A Kuhn, J S Leigh
Jan 1, 1974·Journal of Periodontal Research·T LehnerJ D Manson
Jan 15, 1974·Biochemistry·P Y Chou, G D Fasman
Aug 1, 1974·Oral Surgery, Oral Medicine, and Oral Pathology·C B Sabiston, W A Gold
Aug 1, 1982·Infection and Immunity·S L DotyF N Smith
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D EisenbergT C Terwilliger
Feb 1, 1983·Journal of Clinical Microbiology·A L TruantM T Kelly
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Dec 1, 1981·The Journal of Urology·S RibotH H Eslami

❮ Previous
Next ❯

Citations

Dec 4, 2003·Médecine sciences : M/S·Jean De Rycke, Bernard Ducommun
Aug 8, 2015·Periodontology 2000·Asaf WilenskyLior Shapira
Sep 9, 2010·Infection and Immunity·Christopher W KaplanWenyuan Shi
Oct 2, 2002·Oral Microbiology and Immunology·S Hunt GerardoS Kinder Haake
Mar 13, 2002·Journal of Bacteriology·Vinayak KapatralRoss Overbeek
Oct 8, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Howard K Kuramitsu
Oct 27, 2004·Research in Microbiology·Carmen Helena Barbosa do ValeMaria Auxiliadora Roque de Carvalho

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.