Identification and analysis of Mot3, a zinc finger protein that binds to the retrotransposon Ty long terminal repeat (delta) in Saccharomyces cerevisiae.

Molecular and Cellular Biology
J M MadisonF Winston

Abstract

Spt3 and Mot1 are two transcription factors of Saccharomyces cerevisiae that are thought to act in a related fashion to control the function of TATA-binding protein (TBP). Current models suggest that while Spt3 and Mot1 do not directly interact, they do function in a related fashion to stabilize the TBP-TATA interaction at particular promoters. Consistent with this model, certain combinations of spt3 and mot1 mutations are inviable. To identify additional proteins related to Spt3 and Mot1 functions, we screened for high-copy-number suppressors of the mot1 spt3 inviability. This screen identified a previously unstudied gene, MOT3, that encodes a zinc finger protein. We show that Mot3 binds in vitro to three sites within the retrotransposon Ty long terminal repeat (delta) sequence. One of these sites is immediately 5' of the delta TATA region. Although a mot3 null mutation causes no strong phenotypes, it does cause some mild phenotypes, including a very modest increase in Ty mRNA levels, partial suppression of transcriptional defects caused by a mot1 mutation, and partial suppression of an spt3 mutation. These results, in conjunction with those of an independent study of Mot3 (A. Grishin, M. Rothenberg, M. A. Downs, and K. J. Blu...Continue Reading

References

Oct 2, 1992·Journal of Immunological Methods·J GiaimisP Poindron
Jan 1, 1992·Annual Review of Biochemistry·C O Pabo, R T Sauer
Jan 2, 1992·Gene·T W ChristiansonP Hieter
Jul 1, 1992·Genes & Development·D M EisenmannF Winston
Jul 25, 1992·Nucleic Acids Research·K Robzyk, Y Kassir
Sep 20, 1991·Science·R E Klevit
Jan 1, 1991·Methods in Enzymology·T A KunkelJ McClary
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Sep 15, 1989·Biochemical and Biophysical Research Communications·T Yoshihisa, Y Anraku
Feb 1, 1988·Molecular and Cellular Biology·J N Hirschhorn, F Winston
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Sep 19, 1980·Science·G S RoederG R Fink
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G SimchenG R Fink
Jan 1, 1984·Molecular and Cellular Biology·J L Celenza, M Carlson
Jan 1, 1995·Annual Review of Biochemistry·L Zawel, D Reinberg
Apr 1, 1995·Current Opinion in Genetics & Development·S Y Roth
Jun 1, 1994·Current Opinion in Cell Biology·J A Goodrich, R Tjian
Jan 1, 1994·Trends in Biochemical Sciences·I G Cowell
Dec 1, 1993·Molecular and Cellular Biology·R E Meyers, P A Sharp

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Citations

Nov 15, 2008·PLoS Computational Biology·Anshul KundajeChristina Leslie
Feb 16, 2018·Molecular Biology of the Cell·Patrick A GibneyDavid Botstein
Oct 25, 2006·Molecular Microbiology·Tal LaviSerge Ankri
Jan 11, 2007·Molecular and Cellular Biology·Odeniel SertilCharles V Lowry
Sep 13, 2000·Molecular and Cellular Biology·A J KastaniotisR S Zitomer
Apr 4, 2013·G3 : Genes - Genomes - Genetics·Wei TanGregory Prelich
Aug 31, 2017·Seminars in Cell & Developmental Biology·Risa Burr, Peter J Espenshade
Jun 25, 2015·Microbiology Spectrum·M Joan CurcioPascale Lesage
Aug 19, 2005·Molecular and Cellular Biology·Anne-Laure TodeschiniPascale Lesage

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