Identification and analysis of safener-inducible expressed sequence tags in Populus using a cDNA microarray

Planta
A S RishiArun Goyal

Abstract

Safeners are the chemicals used to protect plants from detrimental effects of herbicides, but their mode of action at the molecular level is not well understood. As an initial step towards understanding the molecular mechanism of safener action in trees, homologous genes in hybrid poplar (Populus nigra x Populus maximowiczii) that were induced by a safener were identified. We here describe the identification of differentially expressed genes in Populus that are induced by Concep-III, a herbicide safener. Expressed sequence tags (ESTs) enriched for transcriptionally induced genes were isolated by suppressive subtractive hybridization (SSH). The SSH library cDNA inserts were used to construct a cDNA microarray for high-throughput validation of the up-regulated expression of safener-induced genes. Single-pass and partial sequences of 1,344 safener-induced ESTs were assembled into 418 singletons and 328 clusters, but the putative functions of almost 53% of the ESTs are not known. Genes encoding proteins involved in all three different phases of safener action, viz., oxidation, conjugation, and sequestration, were found in the SSH library. Almost 75% of genes that showed greater than 2-fold expression upon safener treatment were red...Continue Reading

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Citations

Jul 23, 2011·The Journal of Biological Chemistry·Mark SkipseyRobert Edwards
Dec 7, 2007·BMC Genomics·Tokihiko NanjoKenji Shinohara
Nov 10, 2006·The Plant Journal : for Cell and Molecular Biology·Andreas SjödinJan Karlsson
May 10, 2007·The Plant Journal : for Cell and Molecular Biology·Francisco MerchanMartin Crespi
Feb 6, 2010·Molecular Biology Reports·Hong-Mei YuanJing Jiang

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