Identification and Analysis of the Chloroplast rpoC1 Gene Differentially Expressed in Wild Ginseng

Journal of Pharmacopuncture
Kwang-Ho LeeJun-Hyeog Jang

Abstract

Panax ginseng is a well-known herbal medicine in traditional Asian medicine, and wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention. However, little has actually been reported on the difference between wild ginseng and cultivated ginseng. Thus, to identify and analyze those differences, we used suppressive subtraction hybridization (SSH) sequences with microarrays, realtime polymerase chain reaction (PCR), and reverse transcription PCRs (RT-PCRs). One of the clones isolated in this research was the chloroplast rpoC1 gene, a β subunit of RNA polymerase. Real-time RT-PCR results showed that the expression of the rpoC1 gene was significantly upregulated in wild ginseng as compared to cultivated ginseng, so, we conclude that the rpoC1 gene may be one of the important markers of wild ginseng.

References

Oct 1, 1993·Plant Physiology·J E Mullet
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·L DiatchenkoP D Siebert
Dec 19, 2001·Journal of Korean Medical Science·T K YunH Y Yun
Oct 27, 2005·Journal of Agricultural and Food Chemistry·Wansang LimFrançoise Vermeylen
Jun 28, 2011·Journal of Acupuncture and Meridian Studies·Ki-Rok KwonJun-Hyeog Jang

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Citations

Mar 18, 2015·Journal of Pharmacopuncture·Kwangho LeeKirok Kwon
Mar 1, 2013·Journal of Pharmacopuncture·Han Young-JuJang Jun-Hyeog

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Methods Mentioned

BETA
reverse transcription PCRs
PCRs
PCR

Software Mentioned

Chromas

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