PMID: 8614626Apr 1, 1996Paper

Identification and analysis of the rnc gene for RNase III in Rhodobacter capsulatus

Nucleic Acids Research
R RauhutG Klug

Abstract

The large subunit ribosomal RNA of the purple bacterium Rhodobacter capsulatus shows fragmentation into pieces of 14 and 16S, both fragments forming the functional equivalent of intact 23S rRNA. An RNA-processing step removes an extra stem-loop structure from the 23S rRNA [Kordes, E., Jock, S., Fritsch, J., Bosch, F. and Klug, G. (1994) J. Bacteriol., 176, 1121-1127]. Taking advantage of the fragmentation deficient mutant strain Fm65, we used genetic complementation to find the mutated gene responsible for this aberration. It was identified as the Rhodobacter homologue to mc from Escherichia coli encoding endoribonuclease III (RNase III). The predicted protein has 226 amino acids with a molecular weight of 25.5 kDa. It shares high homology with other known RNase III enzymes over the full length. In particular it shows the double-stranded RNA-binding domain (dsRBD) motif essential for binding of dsRNA substrates. The Fm65 mutant has a frame shift mutation resulting in complete loss of the dsRBD rendering the enzyme inactive. The cloned Rhodobacter enzyme can substitute RNase III activity in an RNase III deficient E. coli strain. Contrary to E. coli, the Rhodobacter mc is in one operon together with the lep gene encoding the lead...Continue Reading

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Citations

Jun 17, 1999·FEMS Microbiology Reviews·A W Nicholson
Jan 26, 2002·The International Journal of Biochemistry & Cell Biology·Christian Conrad, Reinhard Rauhut
Jun 15, 1996·Nucleic Acids Research·G Rotondo, D Frendewey
Nov 20, 2001·Nucleic Acids Research·S JägerG Klug
Jul 21, 2004·Journal of Molecular Microbiology and Biotechnology·Stephanie JägerGabriele Klug
Mar 5, 1999·Molecular Plant-microbe Interactions : MPMI·T WinzerP Müller
Jun 28, 2005·Molecular Microbiology·Elena Evguenieva-Hackenberg
Sep 24, 2004·Biochemical and Biophysical Research Communications·M AmblarC M Arraiano
Nov 5, 1997·Biochemical and Biophysical Research Communications·C A JockN Gavini
Mar 3, 2004·Microbiology·Stephanie JägerGabriele Klug
Mar 21, 1998·FEMS Microbiology Letters·N R Mattatall, K E Sanderson
Oct 3, 2002·The Journal of Biological Chemistry·Elena Evguenieva-HackenbergGabriele Klug
Sep 18, 1997·Microbiology and Molecular Biology Reviews : MMBR·L Thöny-Meyer

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