PMID: 9188589Jul 1, 1997Paper

Identification and analysis of three myristylated vaccinia virus late proteins

Journal of Virology
K H MartinD E Hruby

Abstract

Previous studies have shown that at least three vaccinia virus (VV) late proteins (with apparent molecular asses of 37, 35, and 25 kDa) label with myristic acid. Time course labeling of VV-infected cells with [3H]myristic acid reveals at least three additional putative myristylproteins, with apparent molecular masses of 92, 17, and 14 kDa. The 25-kDa protein has previously been identified as that encoded by the L1R open reading frame, leaving the identities of the remaining proteins to be determined. Sequence analysis led to the preliminary identification of the 37-, 35-, and 17-kDa proteins as G9R, A16L, and E7R, respectively. Using synthetic oligonucleotides and PCR techniques, each of these open reading frames was amplified by using VV DNA as a template and then cloned individually into expression vectors behind T7 promoters. These plasmid constructs were then transcribed in vitro, and the resulting mRNAs were translated in wheat germ extracts and radiolabeled with either [35S]methionine or [3H]myristic acid. Each wild-type polypeptide was labeled with [35S]methionine or [3H]myristic acid in the translation reactions, while mutants containing an alanine in place of glycine at the N terminus were labeled only with [35S]methio...Continue Reading

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Citations

Dec 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tatiana G SenkevichBernard Moss
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·S McCraithS Fields
Jul 18, 2008·The Journal of General Virology·Zhe ZhaoQi-Ya Zhang
Mar 23, 2000·Journal of Virology·C L AfonsoD L Rock
Mar 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hua-Poo SuDavid N Garboczi
Aug 26, 2016·Journal of Virology·Tuan NgoPaul David Gershon
Dec 16, 1998·Journal of Virology·C L AfonsoD L Rock
Mar 19, 2021·Cellular & Molecular Immunology·Bin WangFangfang Zhou
Nov 24, 1999·Virology·C CameronG McFadden
Apr 6, 2001·Seminars in Immunology·J W BarrettG McFadden
Nov 24, 1999·Virology·D O WillerD H Evans
Apr 17, 2002·Journal of Molecular Biology·Sebastian Maurer-StrohFrank Eisenhaber
Jan 20, 2000·Virology·S N ShchelkunovJ J Esposito
Oct 6, 2000·Virology·D W GrosenbachD E Hruby
Jul 7, 1999·Virus Research·K H MartinD E Hruby
Jun 27, 2002·Virology·S N ShchelkunovB Moss
Apr 12, 2003·Analytical Biochemistry·Tsefang S ChenDennis E Hruby
Mar 31, 2004·Trends in Microbiology·Sebastian Maurer-Stroh, Frank Eisenhaber
Sep 29, 2006·Virology·Wolfgang ReschBernard Moss
Dec 13, 2005·Virology Journal·Robert E BlouchDennis E Hruby
Mar 3, 2006·Virology Journal·Jennifer D YoderDennis E Hruby

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