PMID: 9531973Apr 9, 1998Paper

Identification and androgen regulation of egasyn in the mouse epididymis

Biochimica Et Biophysica Acta
A Abou-HaïlaD R Tulsiani

Abstract

The expression and androgen regulation of egasyn, the endoplasmic reticulum-targeting protein of beta-D-glucuronidase, was examined in the mouse-epididymis. The proximal (caput) and distal (corpus & cauda) epididymal tissue extracts were prepared by homogenization and sonication in buffered Triton X-100 solution, and high speed centrifugation. The supernatant when resolved by 2D-PAGE under non-denaturing conditions and stained for esterase activity showed that the distal (but not proximal) epididymis of the normal mouse contain several specific forms of esterases. These forms include a series of four variants (pI 5.2-5.75) with high mobility (HM) and esterase activity, and three faintly staining variants (beginning at pI 6.0) with low mobility (LM). Several lines of evidence indicate that the specific esterases seen in the corpus/cauda epididymidis are egasyn-esterases. Firstly, these molecular forms were not seen in the distal epididymal extracts from the egasyn-deficient mouse. Secondly, the HM forms can be immunoprecipitated with anti-egasyn antibody, suggesting the presence of free egasyn. Finally, the LM forms disappeared after heat treatment (56 degrees C for 8 min), a condition known to dissociate egasyn:beta-D-glucuroni...Continue Reading

References

Dec 1, 1975·The Journal of Cell Biology·E J BrandtR T Swank
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1989·Progress in Nucleic Acid Research and Molecular Biology·K Paigen
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Sep 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·R Ganschow, K Paigen
Aug 1, 1993·Archives of Biochemistry and Biophysics·L ZhenR T Swank

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Citations

Dec 6, 2002·Nature·UNKNOWN Mouse Genome Sequencing ConsortiumEric S Lander

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