Identification and antigenic epitope mapping of immunodominant region amino residues 510 to 672 on the spike protein of the severe acute respiratory syndrome coronavirus

DNA and Cell Biology
Ronghong HuaGuangzhi Tong

Abstract

The severe acute respiratory syndrome (SARS) is a newly emerging human infectious disease caused by the severe acute respiratory syndrome coronavirus (SARS-CoV). The spike (S) protein of SARS-CoV is a major virion structural protein. It plays an important role in the interaction with receptors and neutralizing antibodies. In this study, the S1 domain of the spike protein and three truncated fragments were expressed by fusion with GST in a pGEX-6p-1 vector. Western blot results demonstrated that the 510-672 fragment of the S1 domain is a linear epitope dominant region. To map the antigenic epitope of this linear epitope dominant region, a set of 16 partially overlapping fragments spanning the fragment were fused with GST and expressed. Four antigenic epitopes S1C3 (539-559), S1C4 (548-567), S1C7/8 (583-606), and S1C10/11 (607-630) were identified. Immunization of mice with each of the four antigenic epitope-fused proteins revealed that all four proteins could elicit spike protein specific antisera. All of them were able to bind to the surface domain of the whole spike protein expressed by recombinant baculovirus in insect cells. Identification of antigenic epitopes of the spike protein of SARS-CoV may provide the basis for the d...Continue Reading

References

Feb 13, 2001·Virology·T M Gallagher, M J Buchmeier
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
Apr 25, 2003·Lancet·J S M PeirisUNKNOWN SARS study group
May 6, 2003·Science·Marco A MarraRachel L Roper
Sep 27, 2003·Biochemical and Biophysical Research Communications·Ottavia SpigaNeri Niccolai
Dec 4, 2003·Science·Markus EickmannRino Rappuoli
Dec 13, 2003·The Journal of Biological Chemistry·Swee Kee WongMichael Farzan
Feb 14, 2004·Science·Eliot Marshall, Martin Enserink
Mar 5, 2004·The Journal of Biological Chemistry·Brian TripetRobert S Hodges
Mar 20, 2004·Emerging Infectious Diseases·Bo Jian ZhengShui Shan Lee
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Himani BishtBernard Moss
Jun 9, 2004·Biochemical and Biophysical Research Communications·Ronghong HuaGuangzhi Tong
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ursula J BuchholzPeter L Collins
Jun 29, 2004·Lancet·Jan ter MeulenAlbert D M E Osterhaus
Aug 18, 2004·International Immunology·Naomi TakasukaYasuko Tsunetsugu-Yokota
Aug 21, 2004·The Journal of Infectious Diseases·Yuchun NieMingxiao Ding
Sep 25, 2004·Immunology Letters·Sheng XiongJiang-lin Fan

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