PMID: 7528058Aug 1, 1994Paper

Identification and carbohydrate specificity of a chicken serum mannan-binding protein reactive with a Ra chemotype strain of Salmonella typhimurium

The Journal of Veterinary Medical Science
S Sugii, Y Hirota

Abstract

To compare a chicken serum mannan-binding protein (MBP) with a chicken Ra-reactive factor (RaRF), both serum proteins were isolated by combination of different chromatography. In SDS-PAGE, both purified proteins were resolved into a major protein band with a molecular weight of 34,000 and a few minor protein bands. In Western blotting with both purified proteins, only a single band with a molecular weight of 34,000 was detected with anti-chicken serum MBP antibody, indicating that the major protein band of chicken serum MBP and RaRF are antigenically identical. The combining sites of these two proteins were most reactive with N-acetylmannosamine followed by mannose, N-acetylglucosamine, and L-fucose. These findings suggest that chicken serum MBP may be identical to its corresponding bactericidal factor RaFR in terms of antigenicity and monosaccharide specificity.

Citations

Aug 29, 2002·The Journals of Gerontology. Series B, Psychological Sciences and Social Sciences·Rachel A Pruchno, Dorothy McKenney
Mar 16, 2000·Developmental and Comparative Immunology·S B Laursen, O L Nielsen

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