Identification and cDNA cloning of a new nodule-specific gene, Nms-25 (nodulin-25) of Medicago sativa

Plant Molecular Biology
G B KissJ Soós

Abstract

A new nodule-specific gene, Nms-25 (nodulin-25), was identified in cDNA clones isolated from a nodule-specific cDNA library of Medicago sativa. The first transcript of this gene appeared 9 days after inoculation of the roots with Rhizobium meliloti. The time of expression and the quantity of the transcripts of the Nms-25 gene was similar to that of leghemoglobin genes suggesting a similar regulation. A protein of 246 amino acids could be deduced from a full-length cDNA clone. The first 24 amino acids at the N-terminal end of this protein formed a signal sequence which might direct membrane transport into the peribacteroid space. Using different predictive methods the signal sequence cleaved protein was tentatively predicted to be a water-soluble enzyme, but not hydrolase.

References

Jan 1, 1979·Methods in Enzymology·B Hohn
Oct 1, 1979·Journal of Molecular Evolution·C Sander, G E Schulz
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B Hohn, K Murray
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jan 1, 1975·Molecular Biology, Biochemistry, and Biophysics·R C Burns, R W Hardy
Mar 1, 1988·The Journal of Cell Biology·P PutnokyA Kondorosi
Aug 1, 1988·Computer Applications in the Biosciences : CABIOS·O Gascuel, J L Golmard
Jun 11, 1986·Nucleic Acids Research·G von Heijne
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·P N LewisH A Scheraga
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 1, 1984·Annual Review of Biochemistry·D Eisenberg
Jan 1, 1981·Molecular & General Genetics : MGG·Z BánfalviA Kondorosi
Oct 10, 1980·Nucleic Acids Research·M G Murray, W F Thompson
Jul 13, 1979·Science·R P Legocki, D P Verma

❮ Previous
Next ❯

Citations

Mar 1, 1996·World Journal of Microbiology & Biotechnology·J A MuñozP Ratet
Apr 27, 2002·Physiologia Plantarum·Seppo KaijalainenKristina Lindström
Apr 1, 1993·Molecular & General Genetics : MGG·G B KissL Okrész

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.