Identification and characterisation of a novel inorganic carbon acquisition gene, CIA7, from an insertional mutant of Chlamydomonas reinhardtii

Functional Plant Biology : FPB
Ruby A Ynalvez, James V Moroney

Abstract

Chlamydomonas reinhardtii is a unicellular eukaryotic alga which possesses a CO2-concentrating mechanism (CCM) that enables it to grow at low CO2 concentrations. Previously, insertional mutants were generated to enable isolation of inorganic carbon transporters and other proteins that might be essential for a functional CCM. These mutants have an antibiotic resistance gene that encodes a protein that binds to Zeocin inhibiting Zeocin's DNA strand cleavage activity. The DNA flanking the BleR insert of one of the high CO2 requiring strains, named cia7, was cloned with inverse-PCR and sequenced. Sequence analysis showed homology to conserved bacterial proteins of unknown function, but there were no ESTs in this region of the genome. However, the presence of a gene was established by PCR and RLM-RACE. CIA7 was found to have four exons and the BleR insert was in the fourth exon. CIA7 encodes a protein of 104 amino acids with a calculated molecular mass of 11.3 kDa. Based on the ChloroP prediction program, the protein is predicted to have a chloroplast targeting signal. Complementation analyses results showed possible partially rescued mutants, and RNAi showed several transformants with a sick on low CO2 phenotype with reduced expres...Continue Reading

References

May 25, 1989·Nucleic Acids Research·D Güssow, T Clackson
Jun 14, 1996·The Journal of Biological Chemistry·D M GlerumA Tzagoloff
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 20, 1997·The Journal of Biological Chemistry·V C CulottaJ D Gitlin
Nov 5, 1997·Science·R A PufahlT V O'Halloran
Jul 11, 1998·Science·P G FalkowskiV Smetacek
Mar 27, 2001·Accounts of Chemical Research·A C Rosenzweig
Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·H FukuzawaK Ohyama
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Chung Soon Im, Arthur R Grossman
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Oleg A SineshchekovJohn L Spudich
May 15, 2003·Journal of Cell Science·Bettina KoblenzKarl-Ferdinand Lechtreck
Jan 9, 2004·The Biochemical Journal·Gilles P M BorrellyNigel J Robinson
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Aaron Kaplan, Leonora Reinhold
Oct 27, 2004·The Plant Journal : for Cell and Molecular Biology·Jennifer RohrHeriberto Cerutti
Jan 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·N Sueoka
Jun 15, 2007·Eukaryotic Cell·James V Moroney, Ruby A Ynalvez
Apr 1, 2002·Functional Plant Biology : FPB·Brian ColmanJeffrey S Dason

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Citations

Jun 5, 2010·Photosynthesis Research·John A Raven

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