Identification and characterisation of a biosynthetic locus for Moraxella bovis lipo-oligosaccharide

Carbohydrate Research
Isabelle FaglinIan R Peak

Abstract

Moraxella bovis is a Gram-negative gammaproteobacterium and is one of the causative agents of infectious bovine keratoconjunctivitis. The structure of lipooligosaccharide (LOS) from strain Epp63 was recently elucidated. In the present study a genetic locus of seven encoding genes with high similarity to glycosyltransferases has been identified. Mutation of these putative glycosyltransferase genes resulted in M. bovis mutant bacteria that expressed truncated LOS structures. The structures of the oligosaccharide (OS) expressed by the mutant strains were elucidated and demonstrated the role of the glycosyltransferase enzymes in the LOS biosynthesis of M. bovis. The glycosyltransferase genes designated lgt1, lgt3, and lgt6 are highly similar to the genes in the related bacterium M. catarrhalis. In addition, there are syntenic similarities with the corresponding LOS biosynthesis locus in M. catarrhalis and other members of Moraxellaceae.

References

Feb 16, 2010·Biochemical and Biophysical Research Communications·Isabelle FaglinIan R Peak
Dec 19, 2013·Journal of the American Veterinary Medical Association·Lucas D FunkAnnette M O'Connor
Mar 19, 2014·Carbohydrate Research·Cristina De CastroJennifer C Wilson
Jan 13, 2015·The Veterinary Clinics of North America. Food Animal Practice·John A Angelos

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