Identification and characterisation of barley (Hordeum vulgare) respiratory burst oxidase homologue family members

Functional Plant Biology : FPB
Damien J LightfootAmanda J Able

Abstract

Respiratory burst oxidase homologues (RBOHs) of the human phagocyte gp91phox gene have been isolated from several plant species and the proteins that they encode have been shown to play important roles in the cellular response to biotic stress via the production of superoxide. In this study we have identified and preliminarily characterised six RBOHs from barley (Hordeum vulgare L.). Conservation of the genomic structure and conceptual protein sequence was observed between all six barley RBOH genes when compared with Arabidopsis and rice RBOH gene family members. Four of the six barley RBOH transcripts had wide-spread constitutive spatial expression patterns. The inducible expression profiles of HvRBOHF1 and HvRBOHF2 in response to infection by the necrotrophic fungal pathogens Pyrenophora teres f. teres Drechsler and Rhynchosporium secalis (Oudem) J. Davis were further characterised by quantitative real-time PCR (qPCR). Increased expression of both transcripts was observed in leaf epidermal tissue in response to infection, which is in keeping with a suggested role for both transcripts in the early oxidative burst during the plant response to pathogen invasion. This research provides a basis for further analysis and establishme...Continue Reading

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Citations

Dec 20, 2013·International Journal of Molecular Sciences·Chenxia ChengXiping Wang
Aug 11, 2017·Molecular Plant-microbe Interactions : MPMI·Denise Pereira TorresRalph Hückelhoven
Aug 7, 2010·Molecular Plant-microbe Interactions : MPMI·Reinhard K ProelsRalph Hückelhoven
Mar 19, 2016·Molecular Plant Pathology·Damien J LightfootAmanda J Able
May 8, 2018·Free Radical Research·Wei WangFafu Shen
Oct 27, 2017·Amino Acids·Gurpreet KaurPratap Kumar Pati

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