Identification and characterisation of CYP75A31, a new flavonoid 3'5'-hydroxylase, isolated from Solanum lycopersicum.

BMC Plant Biology
Kristine M OlsenCathrine Lillo

Abstract

Understanding the regulation of the flavonoid pathway is important for maximising the nutritional value of crop plants and possibly enhancing their resistance towards pathogens. The flavonoid 3'5'-hydroxylase (F3'5'H) enzyme functions at an important branch point between flavonol and anthocyanin synthesis, as is evident from studies in petunia (Petunia hybrida), and potato (Solanum tuberosum). The present work involves the identification and characterisation of a F3'5'H gene from tomato (Solanum lycopersicum), and the examination of its putative role in flavonoid metabolism. The cloned and sequenced tomato F3'5'H gene was named CYP75A31. The gene was inserted into the pYeDP60 expression vector and the corresponding protein produced in yeast for functional characterisation. Several putative substrates for F3'5'H were tested in vitro using enzyme assays on microsome preparations. The results showed that two hydroxylation steps occurred. Expression of the CYP75A31 gene was also tested in vivo, in various parts of the vegetative tomato plant, along with other key genes of the flavonoid pathway using real-time PCR. A clear response to nitrogen depletion was shown for CYP75A31 and all other genes tested. The content of rutin and kaem...Continue Reading

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Jul 15, 1974·FEBS Letters·H DiespergerH Sandermann
Jan 1, 1996·Methods in Enzymology·D PomponP Urban
Oct 1, 1996·Journal of the American Dietetic Association·K A Steinmetz, J D Potter
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·N de VettenR Koes
Feb 24, 2001·The Plant Journal : for Cell and Molecular Biology·E T JohnsonG Choi
Mar 23, 2001·Journal of Agricultural and Food Chemistry·S MeiersD Marko
Jul 1, 1995·The Plant Cell·T. A. Holton, E. C. Cornish
Jul 1, 1995·The Plant Cell·R. A. Dixon, N. L. Paiva
Mar 17, 2004·Free Radical Biology & Medicine·Robert J WilliamsCatherine Rice-Evans
Nov 27, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chun Suk JungWalter S De Jong
Dec 18, 2007·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·A AnogianakiP Conti
Mar 6, 2008·Journal of Agricultural and Food Chemistry·Rune SlimestadMichel J Verheul
Jan 1, 2002·The Arabidopsis Book·Danièle Werck-ReichhartSuzanne Paquette
Jan 1, 2007·Metabolomics : Official Journal of the Metabolomic Society·Arnaud BovyRobert D Hall

❮ Previous
Next ❯

Citations

May 10, 2011·Molecular Biology and Evolution·Stacey D Smith, Mark D Rausher
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Apr 30, 2015·Environmental Science and Pollution Research International·Sarvajeet Singh GillNarendra Tuteja
Jun 9, 2014·Plant Science : an International Journal of Experimental Plant Biology·Romain LarbatStéphane Adamowicz
Feb 13, 2015·Applied Biochemistry and Biotechnology·Neha KumariSavita Yadav
Mar 11, 2015·Genome Génome / Conseil National De Recherches Canada·Chunxian ChenFred G Gmitter
Jun 28, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jianan WuQihe Chen
Feb 26, 2016·International Journal of Molecular Sciences·Tian-Shan ZhouYa-Jun Yang
Sep 20, 2017·Journal of Experimental Botany·Takayuki TohgeAlisdair R Fernie
May 18, 2018·FEMS Yeast Research·Michael EichenbergerMichael Naesby
Jun 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuxing DengShanfa Lu
Mar 8, 2020·Bioprocess and Biosystems Engineering·Christian Hernández-GuzmánSergio Huerta-Ochoa
Sep 16, 2018·International Journal of Molecular Sciences·Mariangela CoppolaEmilio Guerrieri
Oct 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yaqiong WuLi-An Xu
Jan 28, 2020·Journal of Agricultural and Food Chemistry·Yun DuMu Li
Aug 1, 2021·The Plant Journal : for Cell and Molecular Biology·Mengyun XingXian Li

❮ Previous
Next ❯

Datasets Mentioned

BETA
DB723744
GQ904194
AY675558

Methods Mentioned

BETA
PCR
transgenic

Software Mentioned

TransMIT
ClustalX
BLAST
qBaseplus

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.