Identification and characterisation of NANOG+/ OCT-4high /SOX2+ doxorubicin-resistant stem-like cells from transformed trophoblastic cell lines

Oncotarget
Reham M BalahmarShiva Sivasubramaniam

Abstract

Treatment of gestational trophoblastic diseases (GTD) involves surgery, radiotherapy and chemotherapy. Although, these therapeutic approaches are highly successful, drug resistance and toxicity remain a concern for high risk patients. This Chemoresistance has also been observed in the presence of cancer stem cells that are thought to be responsible for cases of cancer recurrence. In this study, we report the presence of previously unknown populations of trophoblastic stem-like cells (SLCs) that are resistant to the chemotherapeutic drug doxorubicin. We demonstrate that these populations express the stem cell markers NANOG and Sox2 and higher levels of OCT-4 (NANOG+/OCT-4high/SOX2+). Although chemoresistant, we show that the invasive capacity of these trophoblastic SLCs is significantly inhibited by doxorubicin treatment. To better characterise these populations, we also identified cellular pathways that are involved in SLCs-chemoresistance to doxorubicin. In summary, we provide evidence of the presence of NANOG+/OCT-4+/SOX2+ trophoblastic SLCs that are capable to contribute to the susceptibility to GTD and that may be involved in Chemoresistance associated with drug resistance and recurrence in high risk GTDs' patients. We prop...Continue Reading

References

Dec 1, 1988·Journal of Reproductive Immunology·J N BulmerP M Johnson
Dec 31, 1997·The American Journal of Physiology·F LiuK L Audus
Mar 27, 2004·Reproductive Biology and Endocrinology : RB&E·Rama Soundararajan, A Jagannadha Rao
Sep 21, 2004·Oncogene·Muhammad Al-Hajj, Michael F Clarke
May 4, 2005·Journal of the Society for Gynecologic Investigation·Hui Chen FengSai Wah Tsao
Jun 10, 2008·Developmental Cell·Jing Yang, Robert A Weinberg
Jan 7, 2010·The Journal of Clinical Investigation·Natasha Y FrankMarkus H Frank
Aug 3, 2010·Lancet·Michael J SecklRoss S Berkowitz
Feb 24, 2012·Cell Research·Dean G Tang
Feb 7, 2013·Pharmacology & Therapeutics·Yusuke ShiozawaRussell S Taichman
Sep 4, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M J SecklUNKNOWN ESMO Guidelines Working Group
Apr 3, 2014·International Journal of Oncology·Shuang Li, Qin Li
Mar 17, 2016·Mediators of Inflammation·Bruno SainzHeather L Machado
May 25, 2016·British Journal of Cancer·Patricia SanchoChristopher Heeschen
Jun 28, 2016·Oncotarget·Ruixia Huang, Einar K Rofstad
Oct 28, 2016·Cancer Treatment Reviews·Emilly Schlee VillodreGuido Lenz

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
PCR
electrophoresis

Software Mentioned

Image J
Metacore
OneOmics
SCIEX
Primer
SCIEX OneOmics ( SWATH Proteomics Cloud Toolkit )
Advanced Image Data Analysis
SWATH
Protein Expression Workflow

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved