Identification and characterisation of Simiate, a novel protein linked to the fragile X syndrome

PloS One
Kristin DerligRegina Dahlhaus

Abstract

A strict regulation of protein expression during developmental stages and in response to environmental signals is essential to every cell and organism. Recent research has shown that the mammalian brain is particularly sensitive to alterations in expression patterns of specific proteins and cognitive deficits as well as autistic behaviours have been linked to dysregulated protein expression. An intellectual disability characterised by changes in the expression of a variety of proteins is the fragile X syndrome. Due to the loss of a single mRNA binding protein, the Fragile X Mental Retardation Protein FMRP, vast misregulation of the mRNA metabolism is taking place in the disease. Here, we present the identification and characterisation of a novel protein named Simiate, whose mRNA contains several FMRP recognition motifs and associates with FMRP upon co-precipitation. Sequence analysis revealed that the protein evolved app. 1.7 billion years ago when eukaryotes developed. Applying antibodies generated against Simiate, the protein is detected in a variety of tissues, including the mammalian brain. On the subcellular level, Simiate localises to somata and nuclear speckles. We show that Simiate and nuclear speckles experience specif...Continue Reading

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Citations

May 2, 2014·Frontiers in Cellular Neuroscience·Kristin DerligRegina Dahlhaus
Aug 20, 2016·Environmental Toxicology·Ming-Wei ChaoChia-Yi Tseng
Mar 31, 2018·Frontiers in Molecular Neuroscience·Regina Dahlhaus
Sep 25, 2017·Psychiatry and Clinical Neurosciences·Mary M Y Waye, Ho Yu Cheng

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Methods Mentioned

BETA
Co-immunoprecipitations
transfection
immunoprecipitation
Co-immunoprecipitation

Software Mentioned

Prism
rbαSimiate
E L inear M otif
uadruplex forming G - R ich S equences mapper
ImageJ
Simiate
GraphPad
GST

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