Identification and characterisation of hyaluronate lyase from Streptococcus suis

Microbial Pathogenesis
A G AllenD J Maskell

Abstract

Hyaluronate lyase, which catalyses the degradation of hyaluronic acid (HA), has been described from several pathogenic streptococcal species. We describe, for the first time, identification and purification of hyaluronate lyase from the zoonotic pig pathogen Streptococcus suis. We have cloned the hyaluronate lyase gene from S. suis and used it to generate an allelic replacement knock-out mutant of S. suis serotype 7 that can no longer biosynthesise the enzyme. Interestingly, a limited strain survey indicates that hyaluronate lyase activity is not present in all disease isolates of S. suis. Polyclonal anti-hyaluronate lyase anti-serum raised against our recombinant hyaluronate lyase has been used in Western blots, showing that hyaluronate lyase activity is always associated with the presence of protein of the expected size, whereas lack of hyaluronate lyase activity is due to truncation or absence of the enzyme. We show that hyaluronate lyase activity is required for S. suis to use HA polymer as a carbon source and that supplying exogenous recombinant hyaluronate lyase to all S. suis strains tested allowed fermentation of the resultant HA breakdown products.

References

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Citations

Mar 20, 2014·PloS One·Maria Laura FerrandoJerry M Wells
Apr 19, 2011·Nihon saikingaku zasshi. Japanese journal of bacteriology·Daisuke Takamatsu
Feb 24, 2016·Gut Microbes·Maria Laura Ferrando, Constance Schultsz
Oct 23, 2016·International Journal of Biological Macromolecules·Changliang ZhuXiaolu Jiang
Oct 26, 2005·Cell Biochemistry and Function·K Kemparaju, K S Girish
Aug 19, 2007·Animal Health Research Reviews·Marcelo GottschalkJiangu Xu
Jun 30, 2009·Animal Health Research Reviews·Christoph G Baums, P Valentin-Weigand
Mar 5, 2014·Transboundary and Emerging Diseases·K ManeeratP Srimanote
Nov 24, 2016·Applied Biochemistry and Biotechnology·Changliang ZhuXiaolu Jiang

Related Concepts

Percutaneous Coronary Intervention for ST Elevation Myocardial Infarction-Unstable-Over 12 Hours From Symptom Onset
DNA, Bacterial
Genes, Bacterial
Luronit
Polysaccharide-Lyases
Proteins, Recombinant DNA
Western Blot
Streptococcus suis (bacteria)
Gene Deletion
Sequence Determinations, DNA

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