PMID: 8611142Feb 1, 1996Paper

Identification and characterization of a 44 kDa protein that binds specifically to the 3'-untranslated region of CYP2a5 mRNA: inducibility, subcellular distribution and possible role in mRNA stabilization

The Biochemical Journal
O GenesteM A Lang

Abstract

Stabilization of mRNA is important in the regulation of CYP2a5 expression but the factors involved in the process are not known [Aida and Negishi (1991) Biochemistry 30, 8041-8045]. In this paper, we describe, for the first time, a protein that binds specifically to the 3'-untranslated region of CYP2a5 mRNA and which is inducible by pyrazole, a compound known to increase the half-life of CYP2a5 mRNA. We also demonstrate that pyrazole treatment causes an elongation of the CYP2a5 mRNA poly(A) tail, and that phenobarbital, which is transcriptional activator of the CYP2a5 gene that does not affect the mRNA half-life, neither induces the RNA-binding protein nor affects the poly(A) tail size. SDS/PAGE of the UV-cross-linked RNA-protein complex demonstrated that the RNA-binding protein has an apparent molecular mass of 44 kDa. The protein-binding site was localized to a 70-nucleotide region between bases 1585 and 1655. Treatment of cytoplasmic extracts with an SH-oxidizing agent, diamide, an SH-blocking agent, N-ethylmaleimide or potato acid phosphatase abolished complex-formation, suggesting that the CYP2a5 mRNA-binding protein is subject to post-translational regulation. Subcellular fractionation showed that the 44 kDa protein is pr...Continue Reading

Citations

Jan 9, 1998·Drug Metabolism Reviews·P Honkakoski, M Negishi
May 23, 1998·The Journal of Physiology·D WalkerB H Hirst
Apr 3, 2012·Toxicology and Applied Pharmacology·A'edah Abu-BakarMatti A Lang
May 12, 2004·Chemico-biological Interactions·Guoyu LingXinxin Ding
May 20, 2003·Biochemical Pharmacology·Tina GlisovicFrançoise Raffalli-Mathieu
Jul 8, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A B Robottom-FerreiraL F Ribeiro Pinto
Sep 3, 1998·Molecular Carcinogenesis·U M WastlR Schulte-Hermann
Mar 20, 2002·Molecular Pharmacology·Françoise Raffalli-MathieuMatti A Lang
Sep 25, 2002·The Journal of Biological Chemistry·Arlette MoncionIsabelle De Waziers
Aug 15, 2001·American Journal of Physiology. Cell Physiology·L OliviJ Bressler
Apr 14, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·N SelimT D Boyer
Feb 17, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·A'edah Abu-BakarJukka Hakkola
Dec 25, 2004·The Biochemical Journal·Nhu-Traï TruongIsabelle de Waziers
Mar 17, 2017·BMC Research Notes·Katia S PoçaAna C A X De-Oliveira

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