Identification and characterization of a G-protein regulatory motif in WAVE1

FEBS Letters
Kyoung Seob SongS M Lanier

Abstract

The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate GPR motifs. The WAVE1 GPR peptide inhibited GTPgammaS binding to purified G-protein. Endogenous Gialpha and WAVE1 coimmunoprecipitated from brain lysates. A WAVE1-G-protein complex was also observed following transfection of COS7 cells with Gialpha3 and WAVE1. The docking of Gialpha within a WAVE1 scaffolding complex may facilitate dynamic cycling and/or targeting for efficient and localized control of actin polymerization.

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Citations

Dec 9, 2008·American Journal of Respiratory Cell and Molecular Biology·Kyoung Seob SongJoo-Heon Yoon
Aug 10, 2006·International Journal of Biological Sciences·Francis S Willard
Jan 24, 2007·Pharmacology & Therapeutics·Joe B BlumerStephen M Lanier
Apr 12, 2008·Biochimica Et Biophysica Acta·Oliver VöglerPablo V Escribá
Jun 9, 2016·Scientific Reports·Il-Whan ChoiKyoung Seob Song

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