Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA

Proceedings of the National Academy of Sciences of the United States of America
Tapas K HazraSankar Mitra

Abstract

8-oxoguanine (8-oxoG), ring-opened purines (formamidopyrimidines or Fapys), and other oxidized DNA base lesions generated by reactive oxygen species are often mutagenic and toxic, and have been implicated in the etiology of many diseases, including cancer, and in aging. Repair of these lesions in all organisms occurs primarily via the DNA base excision repair pathway, initiated with their excision by DNA glycosylase/AP lyases, which are of two classes. One class utilizes an internal Lys residue as the active site nucleophile, and includes Escherichia coli Nth and both known mammalian DNA glycosylase/AP lyases, namely, OGG1 and NTH1. E. coli MutM and its paralog Nei, which comprise the second class, use N-terminal Pro as the active site. Here, we report the presence of two human orthologs of E. coli mutM nei genes in the human genome database, and characterize one of their products. Based on the substrate preference, we have named it NEH1 (Nei homolog). The 44-kDa, wild-type recombinant NEH1, purified to homogeneity from E. coli, excises Fapys from damaged DNA, and oxidized pyrimidines and 8-oxoG from oligodeoxynucleotides. Inactivation of the enzyme because of either deletion of N-terminal Pro or Histag fusion at the N terminus...Continue Reading

References

Aug 25, 1992·Nucleic Acids Research·R C de OliveiraC F Menck
Jan 8, 1991·Biochemistry·T J McBrideL A Loeb
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J TchouS Nishimura
Nov 1, 1989·Chemical Research in Toxicology·S J CulpF E Evans
Dec 15, 1987·Biochemistry·Y W Kow, S S Wallace
Jul 11, 1988·Nucleic Acids Research·T R O'ConnorJ Laval
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I MellonP C Hanawalt
Jun 1, 1995·Free Radical Biology & Medicine·A P Breen, J A Murphy
Jan 1, 1994·Pharmacology & Therapeutics·M E GötzM B Youdim
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B N AmesT M Hagen
Jul 1, 1993·Trends in Genetics : TIG·A P Grollman, M Moriya
Feb 1, 1997·Nucleic Acids Research·A KarakayaM Dizdaroglu
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·J P RadicellaS Boiteux
Jan 16, 1999·Biochemistry·M DizdarogluT Roldán-Arjona
Feb 20, 1999·Molecular Cell·A KlunglandT Lindahl
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·A KlunglandD E Barnes
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·O MinowaT Noda
Jul 6, 2000·FEBS Letters·T Lithgow
Jul 6, 2000·FEBS Letters·H E KrokanG Slupphaug
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·F Le PageS Boiteux
Oct 6, 2000·Mutation Research·A DhénautJ P Radicella
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·S E Tsutakawa, P K Cooper
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·S A Leadon

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Citations

Aug 18, 2010·Cellular and Molecular Life Sciences : CMLS·Muralidhar L HegdeSankar Mitra
Jan 13, 2010·Analytical and Bioanalytical Chemistry·Mehjabin KathiwalaAnna Brajter-Toth
Nov 4, 2011·Chromosoma·Angelika L Jacobs, Primo Schär
Jun 5, 2004·DNA Repair·Monica M McTigueCarlos R De Los Santos
Nov 26, 2003·Mutation Research·Jason L Parsons, Rhoderick H Elder
Nov 26, 2003·Mutation Research·Geir SlupphaugHans E Krokan
Nov 26, 2003·Mutation Research·Svein Bjelland, Erling Seeberg
Jun 9, 2005·Mutation Research·Joy L HuffmanJohn A Tainer
Sep 3, 2004·Mutation Research·Mark D EvansMarcus S Cooke
Nov 6, 2003·Toxicology·Markus ChristmannBernd Kaina
Jun 28, 2002·Free Radical Biology & Medicine·Sankar MitraTapas K Hazra
Dec 19, 2002·Free Radical Biology & Medicine·Marcus S CookeMark D Evans
Jul 11, 2003·Free Radical Biology & Medicine·Laura K BancroftRobert S Chapkin
Apr 26, 2003·DNA Repair·Susan S WallaceJeffrey P Bond
Jan 2, 2003·DNA Repair·Harm de WaardGijsbertus T J van der Horst
Apr 26, 2003·DNA Repair·Thomas A RosenquistArthur P Grollman
May 28, 2003·DNA Repair·Holly Miller, Arthur P Grollman
Aug 2, 2003·DNA Repair·Dmitry O ZharkovArthur P Grollman
Dec 25, 2007·Biochemistry·Nirmala KrishnamurthySheila S David
Aug 13, 2013·Journal of the American Chemical Society·Paige L McKibbinSheila S David
Aug 18, 2009·Journal of the American Chemical Society·Ignacio FaustinoModesto Orozco
Aug 18, 2012·Chemical Research in Toxicology·Mark LukinCarlos de Los Santos
Apr 24, 2007·Nature·Irina V KovtunCynthia T McMurray
Jun 22, 2007·Nature·Sheila S DavidSucharita Kundu
Sep 21, 2012·Nature Reviews. Molecular Cell Biology·Lawrence KazakIan J Holt
Feb 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Minmin LiuSusan S Wallace
Nov 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jongchan YeoPeter A Beal
Jul 31, 2013·Proceedings of the National Academy of Sciences of the United States of America·Muralidhar L HegdeSankar Mitra
Jul 25, 2009·The Journal of Biological Chemistry·Kayo ImamuraSylvie Doublié
Dec 21, 2010·The Journal of Biological Chemistry·Dibyendu BanerjeeTapas K Hazra

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