Identification and characterization of a new beta-casein variant in goat milk by high-performance liquid chromatography with electrospray ionization mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Francesco GallianoGiuseppe D'Urso

Abstract

A new variant of beta-casein was detected in the casein fraction obtained from milk of a goat belonging to an autochthonous breed of southern Italy, "Argentata dell'Etna". Reversed-phase high-performance liquid chromatography/electrospray ionization mass spectrometry (RP-HPLC/ESI-MS) analysis indicated that the new beta-casein variant, here named D, has a M(r) 15 Da higher than that of variant C previously described. The modification in the amino acid sequence responsible for the 15 Da difference in M(r) between variants C and D was determined by coupling trypsin digestion with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and RP-HPLC/ESI-MS, and it was demonstrated that it is due to the point mutation Val(207) --> Asn(207). The phosphorylation pattern of the new variant D was shown to be identical to that of variant C, as the protein shows two phosphorylation levels, 5 and 6P, occurring with comparable relative abundances. Ser35 was determined as one of the phosphorylation sites, whereas the others were probably analogous to those determined previously for the beta-Cn variant C, at Thr12 and Ser1517-19. The results reported here indicate that the combined use of RP-HPLC/ESI-MS, MALDI-TOFMS and MS/MS ...Continue Reading

Citations

Nov 9, 2004·Journal of Mass Spectrometry : JMS
Apr 18, 2006·Rapid Communications in Mass Spectrometry : RCM·Vincenzo CunsoloSalvatore Foti
Jul 15, 2006·Journal of Dairy Science·A CaroliG Pagnacco
Oct 19, 2011·European Journal of Mass Spectrometry·Vincenzo CunsoloSalvatore Foti
Jan 2, 2014·Molecular Biology Reports·Maria SelvaggiVincenzo Tufarelli
Mar 13, 2021·Frontiers in Genetics·Siham A RahmatallaGudrun A Brockmann

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