Identification and characterization of a new class of bilin lyase: the cpcT gene encodes a bilin lyase responsible for attachment of phycocyanobilin to Cys-153 on the beta-subunit of phycocyanin in Synechococcus sp. PCC 7002

The Journal of Biological Chemistry
Gaozhong ShenD A Bryant

Abstract

Synechococcus sp. PCC 7002 and all other cyanobacteria that synthesize phycocyanin have a gene, cpcT, that is paralogous to cpeT, a gene of unknown function affecting phycoerythrin synthesis in Fremyella diplosiphon. A cpcT null mutant contains 40% less phycocyanin than wild type and produces smaller phycobilisomes with red-shifted absorbance and fluorescence emission maxima. Phycocyanin from the cpcT mutant has an absorbance maximum at 634 nm compared with 626 nm for the wild type. The phycocyanin beta-subunit from the cpcT mutant has slightly smaller apparent molecular weight on SDS-PAGE. Purified phycocyanins from the cpcT mutant and wild type were cleaved with formic acid, and the products were analyzed by SDS-PAGE. No phycocyanobilin chromophore was bound to the peptide containing Cys-153 derived from the phycocyanin beta-subunit of the cpcT mutant. Recombinant CpcT was used to perform in vitro bilin addition assays with apophycocyanin (CpcA/CpcB) and phycocyanobilin. Depending on the source of phycocyanobilin, reaction products with CpcT had absorbance maxima between 597 and 603 nm as compared with 638 nm for the control reactions, in which mesobiliverdin becomes covalently bound. After trypsin digestion and reverse phase...Continue Reading

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Citations

Oct 4, 2007·Photosynthesis Research·Jun-Ming TuHugo Scheer
Mar 28, 2009·Journal of the American Chemical Society·Jun-Ming TuKai-Hong Zhao
Apr 3, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Xian-Jun WuKai-Hong Zhao
Nov 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Animesh ShuklaDavid M Kehoe
Oct 30, 2009·The Journal of Biological Chemistry·Michaela KupkaKai-Hong Zhao
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May 4, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Lyndsay A CarrigeeWendy M Schluchter

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