Identification and characterization of a novel tospovirus species using a new RT-PCR approach

Archives of Virology
I CortezR Kormelink

Abstract

A novel tospovirus serologically distinct from all established tospovirus species was found in Thailand in Physalis minima L. The S RNA of this virus was cloned by a new RT-PCR approach revealing a nucleotide sequence of 3257 nucleotides. The ambisense RNA segment encoded a nonstructural protein (NSs) of 469 amino acids, with a predicted Mr of 53.2 kDa, and a nucleoprotein (N) of 279 amino acids and a Mr of 31.0 kDa, so far the largest N protein known for any tospovirus species. N protein sequence comparisons revealed closet relationship to the species Watermelon bud necrosis virus (58% identity), Watermelon silver mottle virus and Peanut bud necrosis virus (57%) and a distant relationship to Peanut yellow spot virus (23%) and Peanut chlorotic fanspot virus (22%).

Citations

Jan 12, 2008·Archives of Virology·Pissawan ChiemsombatPreyapan Pongsapich
Mar 6, 2008·Archives of Virology·Jia-Hong DongZhong-Kai Zhang
Feb 13, 2010·Archives of Virology·Afshin Hassani-MehrabanRichard Kormelink
Nov 26, 2010·Archives of Virology·Channarong SeepibanSupat Attathom
Dec 22, 2011·Archives of Virology·Mariana HallwassRenato O Resende
Jan 6, 2012·Archives of Virology·Roger TorresJavier Romero
Oct 24, 2008·Phytopathology·Afshin Hassani-MehrabanRichard Kormelink
Apr 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christoph RudolphJoachim F Uhrig
Dec 4, 2014·Virology Journal·Yueyan YinZhongkai Zhang
Nov 16, 2014·Archives of Virology·A M DullemansR A A van der Vlugt
May 1, 2009·Plant Disease·M CiuffoM Turina
Feb 6, 2017·Archives of Virology·M CiuffoM Turina
Aug 27, 2021·Microbiology Resource Announcements·San Yeong KimChang-Kil Kim

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