Identification and characterization of a novel RNA binding protein that associates with the 5'-untranslated region of the chloroplast psbA mRNA

Biochemistry
Dwight BarnesStephen P Mayfield

Abstract

Binding of proteins to chloroplast-encoded mRNAs has been shown to be an essential part of chloroplast gene expression. Four nuclear-encoded proteins (38, 47, 55, and 60 kDa) have been identified that bind to the 5'-untranslated region of the Chlamydomonas reinhardtii psbA mRNA with high affinity and specificity. We have cloned a cDNA that represents the 38 kDa protein (RB38) and show that it encodes a novel RNA binding protein that is primarily localized within the chloroplast stroma. RB38 contains four 70 amino acid repeats with a high percentage of basic amino acids, as well as an amino-terminal extension predicted to act as a chloroplast import sequence. We demonstrate that the 38 kDa precursor protein is imported into isolated chloroplasts and interacts with high specificity to uridine-rich regions within the 5'-untranslated region of the psbA mRNA. While database searches have identified hypothetical proteins from several other eukaryotic species with high sequence similarity to the deduced amino acid sequence of RB38, no proteins with homology to RB38 have been biochemically characterized. Bioinformatic analysis of the RB38 sequence, together with structure analysis using circular dichroism and protein modeling, suggests...Continue Reading

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Citations

Nov 28, 2012·Applied Microbiology and Biotechnology·Javier A Gimpel, Stephen P Mayfield
Aug 13, 2005·Journal of Molecular Modeling·Christer Levefelt, Dan Lundh
Jul 31, 2007·Photosynthesis Research·Julia Marín-NavarroStephen P Mayfield
Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marco SchottkowskiWilliam Zerges
Jan 24, 2013·Antioxidants & Redox Signaling·Jean-David Rochaix
Dec 7, 2007·The Plant Cell·James Uniacke, William Zerges
Apr 22, 2005·The Plant Journal : for Cell and Molecular Biology·Aravind SomanchiStephen P Mayfield
Mar 19, 2008·Physiologia Plantarum·Lutz WobbeOlaf Kruse
Jun 26, 2012·The Plant Journal : for Cell and Molecular Biology·Christian SchwarzJörg Nickelsen
May 20, 2015·Biochimica Et Biophysica Acta·Yi Sun, William Zerges

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