Identification and characterization of a novel bacterial carbohydrate esterase from the bacterium Pantoea ananatis Sd-1 with potential for degradation of lignocellulose and pesticides

Biotechnology Letters
Qingqing YaoYonghua Zhu

Abstract

Identification and characterization of a novel bacterial carbohydrate esterase (PaCes7) with application potential for lignocellulose and pesticide degradation. PaCes7 was identified from the lignocellulolytic bacterium, Pantoea ananatis Sd-1 as a new carbohydrate esterase. Recombinant PaCes7 heterologously expressed in Escherichia coli showed a clear preference for esters with short-chain fatty acids and exhibited maximum activity towards α-naphthol acetate at 37 °C and pH 7.5. Purified PaCes7 exhibited its catalytic activity under mesophilic conditions and retained more than 40% activity below 30 °C. It displayed a relatively wide pH stability from pH 6-11. Furthermore, the enzyme was strongly resistant to Mg2+, Pb2+, and Co2+ and activated by K+ and Ca2+. Both P. ananatis Sd-1 and PaCes7 could degrade the pesticide carbaryl. Additionally, PaCes7 was shown to work in combination with cellulase and/or xylanase in rice straw degradation. The data suggest that PaCes7 possesses promising biotechnological potential.

References

Oct 18, 2006·Applied Microbiology and Biotechnology·Hyun-Dong Shin, Rachel Ruizhen Chen
May 27, 2014·Journal of Agricultural and Food Chemistry·María Esteban-TorresRosario Muñoz
Sep 10, 2014·Applied Microbiology and Biotechnology·Anatoli TchigvintsevAlexander F Yakunin
Sep 17, 2015·Applied Biochemistry and Biotechnology·Tianxi ZhangShengli Yang
Mar 19, 2016·Environmental Science and Pollution Research International·Ines DhouibMohamed Montassar Lasram
Oct 19, 2016·Biotechnology Advances·Peter BielyVladimír Puchart

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Citations

Jul 3, 2021·International Journal of Environmental Research and Public Health·Ogechukwu Bose ChukwumaNorli Ismail
Aug 24, 2021·3 Biotech·Arpan MukherjeeJay Prakash Verma

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Datasets Mentioned

BETA
AZTE00000000

Methods Mentioned

BETA
PCR
electrophoresis
bioremediation

Software Mentioned

BLASTp
SignalP
MODEL
SWISS
DNAMAN
Clustal
MEGA5
Compute pI / Mw
ExPASy

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