PMID: 8469776Mar 19, 1993Paper

Identification and characterization of a novel angiotensin binding site in cultured vascular smooth muscle cells that is specific for the hexapeptide (3-8) fragment of angiotensin II, angiotensin IV

Regulatory Peptides
K L HallJ W Harding

Abstract

This study demonstrates the existence of a previously unrecognized class of angiotensin binding sites on vascular smooth muscle that exhibit high affinity and specificity for the hexapeptide (3-8) fragment of angiotensin II (AngIV). Binding of [125I]AngIV is saturable, reversible and describes a pharmacologic profile that is distinct and separate from the classic AT1 or AT2 angiotensin receptors. Saturation binding studies utilizing cultured vascular smooth muscle cells obtained from bovine aorta (BVSM) revealed that [125I]AngIV bound to a single high affinity site with an associated Hill coefficient of 0.99 +/- 0.003, exhibiting a KD = 1.85 +/- 0.45 nM and a corresponding Bmax = 960 +/- 100 fmol mg-1 protein. Competition binding curves in BVSM demonstrated the following rank order effectiveness: AngIV > AngII(3-7) > AngIII > Sar1,Ile8 AngII > AngII > AngII(1-7) > AngII(4-8), DuP 753, PD123177. The presence of the non-hydrolyzable GTP analog GTP gamma S, had no effect on [125I]AngIV binding affinity in BVSM. The presence of this novel angiotensin binding site on smooth muscle in high concentration suggests the possibility that this system may play an important, yet unrecognized role in vascular control.

References

Jun 26, 1992·Brain Research·J W HardingJ W Wright
Oct 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K L HallH L Hosick
Dec 1, 1991·Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension·L A SechiM Schambelan
Feb 1, 1991·Trends in Pharmacological Sciences·P B TimmermansW F Herblin
Aug 30, 1989·Biochemical and Biophysical Research Communications·S WhitebreadM de Gasparo
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard

❮ Previous
Next ❯

Citations

Dec 12, 1994·European Journal of Pharmacology·S G BernierG Guillemette
Aug 15, 1996·European Journal of Pharmacology·H C ChampionP J Kadowitz
Jan 1, 1994·Neuroscience and Biobehavioral Reviews·J W Wright, J W Harding
Oct 15, 1995·European Journal of Pharmacology·S G BernierG Guillemette
Feb 11, 1999·Pharmacology, Biochemistry, and Behavior·M Kuziemka-LeskaK Wiśniewski
Oct 9, 2002·Neuroscience and Biobehavioral Reviews·John W WrightJoseph W Harding
Aug 26, 1998·Regulatory Peptides·E A KramárJ W Wright
Nov 1, 1995·The Journal of Clinical Investigation·D M KerinsD E Vaughan
Mar 24, 2006·Cell and Tissue Research·O von Bohlen und Halbach, D Albrecht
Jan 4, 1998·Kidney International·R Ardaillou, D Chansel
Oct 1, 1996·Kidney International·S CzekalskiR Ardaillou
Dec 1, 1994·Kidney International·J W HardingL T Krebs
Sep 29, 2009·Experimental Neurology·John W Wright, Joseph W Harding
May 15, 2004·Progress in Neurobiology·John W Wright, Joseph W Harding
Nov 1, 1994·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·L NäveriJ M Saavedra
Sep 24, 2004·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Gilbert A BurnsJoseph W Harding
Feb 14, 2003·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Georges VauquelinPatrick Vanderheyden
Mar 7, 2002·Journal of the Renin-angiotensin-aldosterone System : JRAAS·T MustafaF A Mendelsohn
Mar 7, 2002·Journal of the Renin-angiotensin-aldosterone System : JRAAS·G PetrescuA Costuleanu
May 1, 1995·Hypertension·M de GasparoP B Timmermans
Dec 2, 2008·CNS Neuroscience & Therapeutics·Dimitri De BundelYvette Michotte
Jan 1, 1995·Journal of Receptor and Signal Transduction Research·L WangP Erne
Nov 11, 1998·Clinical and Experimental Pharmacology & Physiology. Supplement·I MoellerF A Mendelsohn
Oct 1, 1998·The American Journal of Physiology·D ChanselR Ardaillou
Oct 29, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·S ChenE R Block
Jul 4, 2020·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Olive P KhaliqThajasvarie Naicker

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.