PMID: 9557628Apr 29, 1998Paper

Identification and characterization of a porcine torovirus

Journal of Virology
A KronemanH F Egberink

Abstract

A porcine torovirus (PoTV) was identified and characterized; it is a novel member of the genus Torovirus (family Coronaviridae, order Nidovirales), closely related to but clearly distinct from the already recognized equine torovirus (ETV) and bovine torovirus (BoTV) representatives. Immunoelectron microscopy of feces from piglets revealed elongated, 120- by 55-nm particles which were recognized by a torovirus-specific antiserum. Amplification by reverse transcriptase (RT) PCR with primers designed to detect conserved regions (on the basis of the genomes of BoTV strain Breda and ETV strain Berne) resulted in the identification of the 489-bp nucleocapsid gene, encoding a 18.7-kDa protein. The sequence identity in this region between PoTV and both ETV and BoTV was only about 68%, whereas the latter two show 81% identity. Neutralizing antibodies directed against ETV were found in sera of adult and young pigs. In all 10 herds sampled, seropositive animals were present, and 81% of randomly selected adult sows possessed antibodies. A longitudinal study with RT PCR showed that piglets shed virus in the feces for 1 or more days, starting 4 to 14 days after weaning.

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Citations

May 9, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Armando E HoetLinda J Saif
Jun 30, 2005·Animal Health Research Reviews·Armando E Hoet, Linda J Saif
Jun 15, 2013·Tropical Animal Health and Production·Juliane RibeiroAmauri Alcindo Alfieri
Jan 25, 2014·TheScientificWorldJournal·Yuancheng ZhouZhiwen Xu
May 12, 2017·Journal of Virology·Pengcheng ShangYing Fang
Mar 29, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Armando E HoetLinda J Saif
Jun 15, 2007·Clinical and Vaccine Immunology : CVI·Masaki KuwabaraHiroshi Tsunemitsu
Sep 20, 2002·Acta Veterinaria Hungarica·Katalin MatizB Nagy
Jun 7, 2007·The Journal of Veterinary Medical Science·Rikio KirisawaHiroshi Iwai
Jan 10, 2012·Transboundary and Emerging Diseases·X J Meng
Jan 1, 2014·Journal of Veterinary Science·Yong-Il Cho, Kyoung-Jin Yoon
Aug 22, 2003·Reviews in Medical Virology·Saema MagreBirke Bartosch
Feb 4, 2010·Archives of Virology·Dong-Jun ShinKyoung-Oh Cho
Dec 20, 2014·Genome Announcements·Srivishnupriya AnbalaganKaren Schwartz
Jan 3, 2021·Research in Veterinary Science·Nariman ShahhosseiniGary Wong
Jul 22, 1998·The Veterinary Record·L A CornelissenH F Egberink
Apr 4, 2021·Viruses·Makoto Ujike, Fumihiro Taguchi
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Dec 25, 2013·Veterinary Research·Jaime PignatelliDolores Rodríguez
Apr 29, 2011·European Journal of Medicinal Chemistry·Mathew StanleyHansjörg Streicher

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