PMID: 8941773Jun 1, 1996Paper

Identification and characterization of a protease from Streptococcus oralis C104

Oral Microbiology and Immunology
C S Lo, C V Hughes

Abstract

Streptococcus oralis is among the earliest colonizers of the tooth surface during plaque formation. As such, its enzymatic activities may influence ecologic succession on the tooth surface. In the current study, we use zymograms and preparative polyacrylamide gel electrophoresis to identify and purify a protease from S. oralis (sanguis) C104. Proteases from S. oralis C104 were detected in cell pellets at 133, 146 and 176 kDa as clear proteolytic bands on gelatin-substrate zymograms. Preparation of the major (146 kDa) protease were obtained by continuous-elution electrophoresis. The protease was active over the pH range of 7 to 9 with optimum activity between pH 8 and 9. Protease activity was inhibited by several serine protease inhibitors including phenylmethylsulfonyl fluoride, di-isopropyl-phosphofluoridate and aprotinin. The protease showed highest hydrolytic activity against azoalbumin and Bz-Pro-Phe-Arg-NA. Immunofluorescence studies with a polyclonal antiserum to the 146-kDa protease suggest it is present on the cell surface of S. oralis C104. Zymograms of cell pellets from other S. oralis strains as well as S. sanguis and Streptococcus mitis suggest that functionally similar proteases are elaborated by many early coloniz...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Apr 1, 1990·Oral Microbiology and Immunology·A H RogersN J Gully
May 1, 1990·Infection and Immunity·J ReinholdtM Kilian
Dec 1, 1987·Infection and Immunity·D Grenier, B C McBride
Feb 1, 1984·Infection and Immunity·C AnderssonL Linder
Feb 1, 1984·Analytical Biochemistry·P J Kelleher, R L Juliano
Aug 15, 1994·FEMS Microbiology Letters·D J Harrington, R R Russell

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Citations

Aug 6, 2008·Infection and Immunity·Biswendu ChaudhuriFrank A Scannapieco
Jan 1, 1996·Annual Review of Microbiology·C J WhittakerP E Kolenbrander
Jan 18, 2014·Pathogens and Disease·Ilse VandecandelaereTom Coenye
Dec 1, 2007·Microbiology·Christofer KarlssonInga-Maria Frick

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