Identification and characterization of a PutAMT1;1 gene from Puccinellia tenuiflora

PloS One
Yuanyuan BuShenkui Liu

Abstract

Nitrogen is one of the most important limiting factors for plant growth. However, as ammonium is readily converted into ammonia (NH3) when soil pH rises above 8.0, this activity depletes the availability of ammonium (NH4(+)) in alkaline soils, consequently preventing the growth of most plant species. The perennial wild grass Puccinellia tenuiflora is one of a few plants able to grow in soils with extremely high salt and alkaline pH (>9.0) levels. Here, we assessed how this species responds to ammonium under such conditions by isolating and analyzing the functions of a putative ammonium transporter (PutAMT1;1). PutAMT1;1 is the first member of the AMT1 (ammonium transporter) family that has been identified in P. tenuiflora. This gene (1) functionally complemented a yeast mutant deficient in ammonium uptake (2), is preferentially expressed in the anther of P. tenuiflora, and (3) is significantly upregulated by ammonium ions in both the shoot and roots. The PutAMT1;1 protein is localized in the plasma membrane and around the nuclear periphery in yeast cells and P. tenuiflora suspension cells. Immunoelectron microscopy analysis also indicated that PutAMT1;1 is localized in the endomembrane. The overexpression of PutAMT1;1 in A. tha...Continue Reading

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Citations

Jul 30, 2014·Applied Microbiology and Biotechnology·Anita EmmerstorferHarald Pichler
Jun 7, 2019·Plant Signaling & Behavior·Yuanyuan BuShenkui Liu
Sep 10, 2018·Functional & Integrative Genomics·Tiago Benedito Dos SantosDouglas S Domingues
May 24, 2020·International Journal of Molecular Sciences·Dong-Li HaoYan-Hua Su
Aug 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Adam MonierThomas A Richards

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Datasets Mentioned

BETA
JQ279059

Methods Mentioned

BETA
transgenic
PCR
electron microscopy
electrophoresis

Software Mentioned

Molecular Evolutionary Genetics Analysis ( MEGA )
MEGA
CLUSTALX

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