Identification and characterization of a second superoxide dismutase gene (sodM) from Staphylococcus aureus

Journal of Bacteriology
Michelle Wright Valderas, Mark E Hart

Abstract

A gene encoding superoxide dismutase (SOD), sodM, from S. aureus was cloned and characterized. The deduced amino acid sequence specifies a 187-amino-acid protein with 75% identity to the S. aureus SodA protein. Amino acid sequence comparisons with known SODs and relative insensitivity to hydrogen peroxide and potassium cyanide indicate that SodM most likely uses manganese (Mn) as a cofactor. The sodM gene expressed from a plasmid rescued an Escherichia coli double mutant (sodA sodB) under conditions that are otherwise lethal. SOD activity gels of S. aureus RN6390 whole-cell lysates revealed three closely migrating bands of activity. The two upper bands were absent in a sodM mutant, while the two lower bands were absent in a sodA mutant. Thus, the middle band of activity most likely represents a SodM-SodA hybrid protein. All three bands of activity increased as highly aerated cultures entered the late exponential phase of growth, SodM more so than SodA. Viability of the sodA and sodM sodA mutants but not the sodM mutant was drastically reduced under oxidative stress conditions generated by methyl viologen (MV) added during the early exponential phase of growth. However, only the viability of the sodM sodA mutant was reduced when...Continue Reading

References

Dec 1, 1991·Microbiological Reviews·S B Farr, T Kogoma
Jun 3, 1988·Science·J A Imlay, S Linn
Feb 1, 1985·American Journal of Epidemiology·R W HaleyT G Emori
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Jul 1, 1983·Applied and Environmental Microbiology·D W Dyer, J J Iandolo
Jan 1, 1995·Annual Review of Biochemistry·I Fridovich
Dec 29, 1995·Gene·A M Guérout-FleuryP Stragier
Jul 23, 1999·Infection and Immunity·J L SnodgrassM S Smeltzer
Jan 1, 1997·Plant Physiology·J. L. DonahueR. G. Alscher

❮ Previous
Next ❯

Citations

Nov 4, 2011·Seminars in Immunopathology·James E Cassat, Eric P Skaar
Nov 7, 2002·Trends in Microbiology·Malcolm J HorsburghSimon J Foster
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Steven M DamoWalter J Chazin
May 8, 2013·The Journal of Antimicrobial Chemotherapy·Rabia LadjouziAxel Hartke
Mar 17, 2009·Journal of Bacteriology·Anand Ballal, Adhar C Manna
Dec 21, 2007·Journal of Clinical Microbiology·Muzaffar HussainKarsten Becker
Apr 14, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuya MorikawaKunio Takeyasu
Jul 29, 2010·Molecular Systems Biology·Amardeep KaurNitin S Baliga
Jul 3, 2015·Molecular Microbiology·Zuelay Rosario-CruzJeffrey M Boyd
Apr 4, 2015·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Jacqueline R MoreyThomas E Kehl-Fie
Oct 3, 2003·Microbiology·Michail H KaravolosSimon J Foster
Jul 18, 2017·Frontiers in Cellular and Infection Microbiology·Fermin E GuerraJovanka M Voyich
Aug 21, 2019·Journal of Bacteriology·Shanti PandeyMohamed O Elasri
Dec 5, 2019·Microorganisms·Kazuya MorikawaKunio Takeyasu
Feb 6, 2021·Biological Chemistry·Nico LinznerHaike Antelmann
Mar 31, 2019·Microbiology Spectrum·Nienke W M de JongJos A G van Strijp

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.