Identification and characterization of a squamate reptilian amelogenin gene: Iguana iguana

Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
X S WangThomas G H Diekwisch

Abstract

As the principal components of the developing tooth enamel matrix, amelogenins play a significant role in tooth enamel formation and organization. In order to elucidate the structure and function of amelogenins in the evolution of enamel, we have selected the Iguana iguana as a squamate model organism. Here we report the first complete squamate amelogenin sequence available as of yet and document unique features of Iguana amelogenins and enamel. Transmission electron microscopy documented randomly oriented Iguana enamel crystals during the elongation phase compared with organized enamel crystal patterns at comparable stages in mammals. Sequencing of PCR amplified products revealed a full-length I. iguana amelogenin cDNA containing 877 nucleotides with a 564 nucleotide coding sequence encoding 187 amino acids. The homologies of the newly discovered I. iguana amelogenin amino acid sequence with the published mouse, caiman (Palaeosuchus), and snake (Elaphe) amelogenin were 41.3%, 53.5%, and 55.5%, respectively. On Western blots one major protein with a molecular weight of 24 kDa, and two minor proteins with molecular weights of 28 and 13.5 kDa, respectively, were detected based on the cross-reactivity of antisera against recombina...Continue Reading

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Citations

Sep 29, 2011·Journal of Dental Research·J-Y SireP K Denbesten
Jan 25, 2012·European Journal of Oral Sciences·Xuanyu LuThomas G H Diekwisch
Jul 14, 2007·Cells, Tissues, Organs·Jean-Yves SireXun Gu
Sep 21, 2011·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Gregory R Handrigan, Joy M Richman
May 6, 2008·Developmental Biology·Marcela BuchtováJoy M Richman

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