Identification and Characterization of a Stem Cell-Like Population in Bovine Milk: A Potential New Source for Regenerative Medicine in Veterinary

Stem Cells and Development
Caterina PipinoGiuseppe Martino

Abstract

Milk is a complex fluid required for development, nutrition and immunological protection to the newborn offspring. Interestingly, latest finding proved the presence of novel stem cell population in human milk with multilineage differentiation potential. Given that little is known about cellular milk content in other mammalian species such as bovine, the purpose of our study was to isolate and characterize a potential stem cell-like population in bovine milk. In detail, we first analyzed the phenotype of the isolated cells able to grow in plastic adherence and then their capability to differentiate into osteogenic, chondrogenic, and adipogenic lineages. Bovine milk stem cells (bMSCs) resulted plastic adherent and showed a heterogeneous population with epithelial and spindle-shaped cells. Successively, their immunophenotype indicated that bovine milk cells were positive for the typical epithelial markers E-cadherin, cytokeratin-14, cytokeratin-18, and smooth muscle actin. Notably, a subset (30%-40%), constantly observed in purified milk cells, showed the typical mesenchymal surface antigens CD90, CD73, and CD105. Furthermore, the same percentage of bMSCs expressing CD90, CD73, and CD105 presented the stemness markers SOX2 and OCT...Continue Reading

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Citations

Jul 29, 2020·In Vitro Cellular & Developmental Biology. Animal·Chen YijiaoAn Junhui
Dec 28, 2018·Antioxidants·Mario BarattaEugenio Martignani
Mar 25, 2020·International Journal of Molecular Sciences·Danilo BucaPaola Lanuti
Apr 9, 2020·International Journal of Molecular Sciences·Pasquale SimeoneRenato Mariani-Costantini
Apr 12, 2019·Human Cell·Natalia NinkinaVladimir L Buchman
Dec 4, 2020·International Journal of Molecular Sciences·Mirthe DekkerDominique P V de Kleijn

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Methods Mentioned

BETA
FACS
flow cytometry
reverse transcription-PCR
light microscopy
SMA

Software Mentioned

FACSuite
IDEAS
ImageStream
AMNIS

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