Identification and characterization of a UDP-D-glucuronate 4-epimerase in Arabidopsis

FEBS Letters
Björn UsadelMarkus Pauly

Abstract

One of the major sugars present in the plant cell wall is d-galacturonate, the dominant monosaccharide in pectic polysaccharides. Previous work indicated that one of the activated precursors necessary for the synthesis of pectins is UDP-d-galacturonate, which is synthesized from UDP-d-glucuronate by a UDP-d-glucuronate 4-epimerase (GAE). Here, we report the identification, cloning and characterization of a GAE6 from Arabidopsis thaliana. Functional analysis revealed that this enzyme converts UDP-d-glucuronate to UDP-d-galacturonate in vitro. An expression analysis of this epimerase and its five homologs in the Arabidopsis genome by quantitative RT-PCR and promoter::GUS fusions indicated differential expression of the family members in plant tissues and expression of all isoforms in the developing pollen of A. thaliana.

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Citations

Jul 1, 2009·Journal of Biochemistry·Xiaogang GuMaor Bar-Peled
Nov 21, 2009·Plant Physiology·Bryan W PenningNicholas C Carpita
Jun 29, 2010·Applied and Environmental Microbiology·Quan WangLei Wang
Mar 24, 2016·Plant Molecular Biology·Valentino RuggieriMaria Luisa Chiusano
Apr 4, 2006·Analytical Biochemistry·Takao OhashiSumihiro Hase
Nov 12, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sen YinJian-Qiang Kong
Mar 6, 2018·The Plant Journal : for Cell and Molecular Biology·Adam M Saffer, Vivian F Irish
Apr 29, 2006·The Journal of Biological Chemistry·Christine BarberGeorg J Seifert
Jun 5, 2018·International Journal of Molecular Sciences·Xing DingXingguo Zhang
Mar 12, 2021·Plant Science : an International Journal of Experimental Plant Biology·L Medina-PucheR Blanco-Portales

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