PMID: 8939027Nov 1, 1996Paper

Identification and characterization of a mobilizing plasmid, pND300, in Lactococcus lactis M189 and its encoded nisin resistance determinant

The Journal of Applied Bacteriology
K DuanN W Dunn

Abstract

A 60 kb conjugative plasmid, pND300, which encodes nisin resistance, was identified in Lactococcus lactis ssp. lactis (L. lactis) M189. pND300 was found to mobilize the transfer of some other plasmids as indicated by the mobilization of plasmids encoding lactose utilization. The nisin resistance determinant from pND300 was initially subcloned on a 12 kb DNA fragment and subsequently reduced to 10.4 kb. Restriction analysis, PCR, Southern hybridization and sequencing illustrated that the nisin resistance of pND300 is very similar to that encoded by the transposon involved in nisin production. pND300 encodes nisR as well as nisK and the recently reported nisF, nisE and nisG, but does not encode nisI. The DNA fragment encoding the nis genes is flanked by IS946 with a copy at each end in reverse orientation. The expression of these nis genes is probably controlled by a putative promoter upstream of nisR, which is composed of the TTGCAA hexanucleotide on the insertion sequence IS946 and the TATAAT sequence 21 bp downstream.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Feb 1, 1992·FEMS Microbiology Reviews·M van de GuchteG Venema
Oct 1, 1987·Applied and Environmental Microbiology·D A RomeroA Mercenier
Jan 1, 1985·Applied and Environmental Microbiology·M L Dao, J J Ferretti
Sep 1, 1983·Antonie van Leeuwenhoek·L L McKay
Sep 1, 1983·Applied and Environmental Microbiology·D G Anderson, L L McKay
Mar 1, 1995·Applied and Environmental Microbiology·K Siegers, K D Entian
Mar 1, 1994·Applied and Environmental Microbiology·G EngelkeK D Entian
Mar 1, 1988·Applied and Environmental Microbiology·Ian B PowellBarrie E Davidson

❮ Previous
Next ❯

Citations

Jan 5, 2002·International Journal of Food Microbiology·J ClevelandM L Chikindas
Feb 11, 2004·FEMS Microbiology Letters·Olli KoponenPer E J Saris
Apr 9, 2014·Antimicrobial Agents and Chemotherapy·Harsh MathurR Paul Ross
Oct 27, 2001·Applied and Environmental Microbiology·G DaiN W Dunn
Mar 23, 2021·Frontiers in Bioengineering and Biotechnology·Lieke A van GijtenbeekGunnar Oregaard

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.