Identification and characterization of a nucleolar phosphoprotein, Nopp140, as a transcription factor.

Molecular and Cellular Biology
L H MiauS C Lee

Abstract

Expression of the alpha-1 acid glycoprotein (AGP) gene (agp) is activated by a key transcription factor, AGP/enhancer-binding protein (AGP/EBP, commonly called C/EBP beta), in the liver during the acute-phase response. In addition to this positive regulation, agp is negatively regulated by nucleolin (T. H. Yang et al., Mol. Cell. Biol. 14:6068-6074, 1994). Other factors involve in positive regulation of the agp gene are poorly characterized. In a systematic search for factors that may interact with AGP/EBP, we have identified Nopp 140, a phosphoprotein of 140 kDa, by immunoaffinity chromatography. Nopp 140 not only functions as a transcriptional activator per se but also interacts with AGP/EBP to synergistically activate the agp gene in an AGP/EBP-binding motif-dependent manner. In addition to interacting with AGP/EBP, Nopp140 interacts specifically with TFIIB. Distinct regions of Nopp140 that interact with AGP/EBP and TFIIB have been characterized. The sequence of Nopp140 contains several stretches of serine- and acidic amino acid-rich sequences which are also found in ICP4 of herpes simplex virus type 1, a known transcription factor that interacts with TFIIB. The physical interaction between TFIIB and wild-type Nopp140 or sev...Continue Reading

References

Jun 1, 1992·Immunological Reviews·S Akira, T Kishimoto
Jul 10, 1992·Cell·U T Meier, G Blobel
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S KinoshitaT Kishimoto
Dec 1, 1990·The Journal of Cell Biology·U T Meier, G Blobel
Jul 1, 1988·Genes & Development·W H LandschulzS L McKnight
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M Sawadogo, R G Roeder
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J L VanniceG M Ringold
Feb 1, 1994·Immunology Today·H Baumann, J Gauldie
Jun 1, 1995·Current Opinion in Cell Biology·F Melchior, L Gerace
Dec 1, 1994·The Journal of Cell Biology·U T Meier, G Blobel
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·H GeR G Roeder
Feb 24, 1995·Cell·D Thanos, T Maniatis
Feb 1, 1996·Journal of Virology·K XiaD M Knipe
Dec 1, 1995·Trends in Biochemical Sciences·L Guarente

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Citations

Feb 18, 2015·Journal of Cellular Physiology·Ronit Salomon-KentGordon L Hager
Jun 23, 2009·The American Journal of Pathology·Yu-Chyi HwangChin-Tarng Lin
Sep 16, 2008·Biochemical and Biophysical Research Communications·Won-Kyu LeeYeon Gyu Yu
Nov 21, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Nadine KosslerArno Kalkuhl
Jan 22, 2003·The Plant Journal : for Cell and Molecular Biology·Julien CurabaGilles Vachon
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·M I Tussié-LunaA L Roy
Aug 20, 2005·American Journal of Respiratory and Critical Care Medicine·Li ZhongEdward A Hirschowitz
Jun 16, 2006·Cell Research·Szecheng J LoHuey-Jen Lai
Jun 26, 2001·Molecular Reproduction and Development·V BaranJ E Flechon
Jan 26, 2002·Molecular Biology of the Cell·John M Waggener, Patrick J DiMario
Mar 30, 2007·Molecular Biology of the Cell·Zhengfang Cui, Patrick J DiMario
Apr 13, 2007·Molecular Biology of the Cell·Nupur KitturU Thomas Meier
Mar 17, 2005·Chromosoma·U Thomas Meier
Jul 5, 2008·Journal of Cell Communication and Signaling·Morten I Rasmussen, Denys N Wheatley
Apr 22, 2009·Histochemistry and Cell Biology·Marc ThiryDominique Ploton
Jun 17, 2000·The Journal of Biological Chemistry·M Baer, P F Johnson
May 2, 2003·The Journal of Biological Chemistry·Yan-Hsiung WangSheng-Chung Lee
Dec 19, 2020·Molecular & Cellular Proteomics : MCP·Xiao-Ting WenYong-Zhe Li
Feb 6, 2021·Molecular & Cellular Proteomics : MCP·Xiaoting WenYongzhe Li
Sep 23, 1997·Biochemical and Biophysical Research Communications·W A PaznekasE W Jabs
Oct 11, 2003·Protein Expression and Purification·Yun-Kyeong KimYeon Gyu Yu
May 10, 2002·European Journal of Cell Biology·Xin SunAlla T Alzhanova-Ericsson

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