Identification and characterization of a constitutive HSP75 in sea urchin embryos

Biochemical and Biophysical Research Communications
G SconzoG Ghersi

Abstract

An antiserum against a hsp of the 70-kDa family was prepared, by means of a fusion protein, which was able to detect a constitutive 75-kDa hsc in the sea urchin P. lividus. This hsc was present both during oogenesis and at all developmental stages. A two-dimensional electrophoresis has revealed four isolectric forms of this 75-kDa hsc. The amino acid sequence of the fragment used to prepare the anti-hsp70 antibodies revealed a 43% identity with the corresponding part of sea urchin sperm receptor, and in mature eggs a brighter immunofluorescence was seen all around the cell cortex where the receptor for sea urchin sperm is localized. In oocytes the hsp75 was localized in the cytoplasms but not in the nuclei. In the embryos a higher hsp75 concentration was found in the portion facing the lumen of the cells which invaginate at gastrulation.

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Citations

Aug 31, 1999·Development, Growth & Differentiation·G GiudiceM C Roccheri
Oct 11, 2003·Biochemical and Biophysical Research Communications·Fabiana GeraciGabriella Sconzo
Sep 1, 2004·Biochemical and Biophysical Research Communications·Fabiana GeraciGabriella Sconzo
Jun 15, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Tal MizrahiZeev Arad
Sep 11, 2004·Biochemical and Biophysical Research Communications·Maria Carmela RoccheriValeria Matranga
Dec 22, 1999·Cell Motility and the Cytoskeleton·R E Stephens, N A Lemieux
Jul 25, 2007·Development Genes and Evolution·Helen M Gunter, Bernard M Degnan
Apr 19, 2008·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Helen M Gunter, Bernard M Degnan
Jun 27, 2014·European Journal of Dentistry·Sofi Beaula WinfredGanesh Venkatraman
Oct 30, 2014·Human & Experimental Toxicology·Zhengli YanTianbao Zhang
Dec 6, 1997·Biochemical and Biophysical Research Communications·G SconzoR Sirchia

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