PMID: 9422602Jan 9, 1998Paper

Identification and characterization of aarF, a locus required for production of ubiquinone in Providencia stuartii and Escherichia coli and for expression of 2'-N-acetyltransferase in P. stuartii

Journal of Bacteriology
D R MacingaP N Rather

Abstract

Providencia stuartii contains a chromosomal 2'-N-acetyltransferase [AAC(2')-Ia] involved in the O acetylation of peptidoglycan. The AAC(2')-Ia enzyme is also capable of acetylating and inactivating certain aminoglycosides and confers high-level resistance to these antibiotics when overexpressed. We report the identification of a locus in P. stuartii, designated aarF, that is required for the expression of AAC(2')-Ia. Northern (RNA) analysis demonstrated that aac(2')-Ia mRNA levels were dramatically decreased in a P. stuartii strain carrying an aarF::Cm disruption. The aarF::Cm disruption also resulted in a deficiency in the respiratory cofactor ubiquinone. The aarF locus encoded a protein that had a predicted molecular mass of 62,559 Da and that exhibited extensive amino acid similarity to the products of two adjacent open reading frames of unknown function (YigQ and YigR), located at 86 min on the Escherichia coli chromosome. An E. coli yigR::Kan mutant was also deficient in ubiquinone content. Complementation studies demonstrated that the aarF and the E. coli yigQR loci were functionally equivalent. The aarF or yigQR genes were unable to complement ubiD and ubiE mutations that are also present at 86 min on the E. coli chromos...Continue Reading

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May 2, 2006·Molecular and Cellular Biology·Robyn BranickySiegfried Hekimi
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Oct 1, 2003·The Journal of Biological Chemistry·Jason BurgessSiegfried Hekimi
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Dec 3, 2016·Frontiers in Microbiology·Dennis VersluisMark W J van Passel
Mar 8, 2005·The Journal of Veterinary Medical Science·Waren N BaticadosChihiro Sugimoto
Mar 6, 1999·Current Opinion in Microbiology·K Bush, G H Miller
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Aug 15, 2006·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·R A Proctor, A von Humboldt
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Mar 6, 2008·American Journal of Human Genetics·Clotilde Lagier-TourenneMichel Koenig

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