PMID: 6411466Aug 15, 1983Paper

Identification and characterization of an UDP-Gal: N-acetyllactosaminide alpha-1,3-D-galactosyltransferase in calf thymus

European Journal of Biochemistry
D H Van den EijndenW E Schiphorst

Abstract

Calf thymus was found to contain a high activity of a galactosyltransferase, which transfers galactose from UDP-galactose to asialo-alpha 1-acid glycoprotein and N-acetyllactosamine. By means of competition and acceptor-specificity studies the enzyme could be distinguished from an N-acetylglucosaminide beta-1,4-galactosyltransferase and an N-acetylgalactosamine-protein beta-1,3-galactosyltransferase, which in addition occur in calf thymus, as well as from the blood-group-B-associated alpha-galactosyltransferase. Identification of the products revealed that the enzyme accomplishes an alpha 1 leads to 3 linkage resulting in a terminal Gal(alpha 1 leads to 3)Gal(beta 1 leads to 4)GlcNAc sequence. The enzyme is membrane-bound and is activated by Triton X-100. It shows optimal activity over a broad pH range (5.5-7.0) and has a pronounced requirement for Mn2+ ions (Km = 6.1 mM) for its action. It is suggested that the alpha-1,3-galactosyltransferase functions in the biosynthesis of calf thymocyte cell-surface glycoconjugates including glycoproteins.

References

Apr 1, 1979·European Journal of Biochemistry·D H van den EijndenW E Schiphorst
Oct 11, 1967·Biochemical and Biophysical Research Communications·D ZidermanW M Watkins
Jan 31, 1972·Biochemical and Biophysical Research Communications·V M HearnW M Watkins
Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·K BrewR L Hill
Jul 26, 1968·Biochemical and Biophysical Research Communications·A KobataV Ginsburg
Jan 14, 1983·Biochemical and Biophysical Research Communications·H Van HalbeekD H Van den Eijnden
Jan 1, 1980·Advances in Carbohydrate Chemistry and Biochemistry·J Montreuil
Jan 1, 1982·European Journal of Biochemistry·G SpikJ F Vliegenthart
Jan 1, 1982·Methods in Enzymology·K YamashitaA Kobata
Jul 1, 1982·European Journal of Biochemistry·A Pierce-CretelJ F Vliegenthart
May 1, 1968·The Biochemical Journal·C RaceW M Watkins

❮ Previous
Next ❯

Citations

Jan 24, 2004·Surgery·Jay DoucetVivian C McAlister
Mar 1, 1985·Analytical Biochemistry·W M BlankenD H van den Eijnden
Feb 14, 1997·The Journal of Biological Chemistry·S Y Mir-ShekariI T Schulze

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.