Identification and characterization of an exotoxin from Staphylococcus aureus associated with toxic-shock syndrome

The Journal of Infectious Diseases
P M SchlievertR D Nishimura

Abstract

Toxic-shock syndrome (TSS) is believed to be caused by a toxin produced by Staphylococcus aureus. An exotoxin has been identified that is associated with strains of S. aureus isolated from patients with TSS. Coded strains of S. aureus were tested for the presence of the exotoxin by polyacrylamide gel isoelectric focusing. Sixty isolates of S. aureus were tested; 28 (100%) of 28 isolates from patients with TSS but only five (16%) of 32 control isolates produced the toxin (P much less than 0.001). This protein exotoxin, which was purified by differential precipitation with ethanol and thin-layer isoelectric focusing, had an isoelectric point of 7.2. When tested by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the toxin migrated as a homogeneous protein with a molecular weight of 22,000. The exotoxin produced fever in rabbits and enhanced susceptibility to lethal shock caused by endotoxin. Other biologic properties of the exotoxin included lymphocyte mitogenicity and the capacity to suppress synthesis of IgM antibody to sheep erythrocytes.

Citations

Jan 1, 1983·Molecular & General Genetics : MGG·L P Johnson, P M Schlievert
Jan 1, 1994·Aesthetic Plastic Surgery·C A RheeI T Jackson
Jul 15, 1986·Klinische Wochenschrift·N Dickgiesser, B Kustermann
Jan 4, 1988·Klinische Wochenschrift·N Dickgiesser, F Düzgün
Jan 1, 1994·Immunologic Research·J M Green, C B Thompson
Apr 22, 1988·Journal of Immunological Methods·T A KuffnerB D Plikaytis
Jul 1, 1996·Molecular Immunology·R MakidaD Y Leung
Jan 1, 1982·Pharmacology & Therapeutics·J H Freer, J P Arbuthnott
Oct 1, 1995·International Journal of Immunopharmacology·K ImanishiT Uchiyama
Jul 1, 1988·FEMS Microbiology Immunology·J C de AzavedoJ P Arbuthnott
Mar 9, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Hirotsugu KanoJun Abe
Feb 22, 1986·Lancet·J DevenishJ Goold
Jun 6, 2000·Advanced Drug Delivery Reviews·B PerssonR K Alpaugh
Jan 1, 1989·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·A S NaiduT Wadström
Aug 8, 1998·The Journal of Emergency Medicine·D DavisE J Heffernan
Jan 21, 2000·FEMS Immunology and Medical Microbiology·H I DaghistaniA A Shehabi
Oct 1, 1995·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·F Schumacher-PerdreauG Pulverer
Feb 1, 1985·The Journal of Hygiene·A K MelconianY Brun
Feb 16, 2006·Biochemistry·Marnie L Peterson, Patrick M Schlievert
Nov 18, 2008·Biochemistry·Amanda J BrosnahanPatrick M Schlievert
Jul 17, 2010·Nature Reviews. Microbiology·Richard P NovickJose R Penadés
Jun 25, 1981·The New England Journal of Medicine·D M BellJ F Modlin
May 31, 1984·The New England Journal of Medicine·J N Sheagren
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P SchollT Chatila
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J M MusserR K Selander
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A Zembowicz, J R Vane
Mar 1, 1988·The Journal of Experimental Medicine·C JupinM Parant
Sep 1, 1990·The Journal of Experimental Medicine·Y ChoiB L Kotzin
Jan 1, 1992·The Journal of Experimental Medicine·N BhardwajA J Nisanian
Feb 1, 1992·The Journal of Experimental Medicine·J W KapplerP Marrack
Aug 1, 1994·The Journal of Experimental Medicine·A R HamadJ W Kappler
Nov 1, 1994·The Journal of Experimental Medicine·K RenJ B Zabriskie

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