Abstract
We recently reported that interleukin-3 (IL-3) stimulation of the murine IL-3-responsive cell line, B6SUtA1, results in the rapid phosphorylation of the beta subunit of the IL-3 receptor (IL-3R), not only on tyrosine residues but on serine/threonine (Ser/Thr) residues as well. Since this occurred even at 4 degrees C, it suggested that a Ser/Thr-specific kinase might be closely associated with the IL-3R. To test this possibility, IL-3R complexes were isolated with anti-IL-3R (alpha IL-3R) antibodies, and in vitro phosphorylation studies were undertaken. These revealed the presence of a 110-kDa protein that was heavily phosphorylated in vitro on serine and threonine residues and that bound selectively to gamma-ATP-Sepharose beads. Moreover, this protein, which was not the 110-kDa subunit of phosphatidylinositol 3-kinase, was tyrosine phosphorylated in response to IL-3 and was specifically labeled in vitro with azido-[32P]ATP. These data, together with in vitro kinase inhibitor studies, suggest that an as yet uncharacterized H7- and staurosporine-sensitive 110-kDa Ser/Thr kinase may be constitutively associated with the IL-3R and activated following IL-3 stimulation. A comparison of IL-3R and erythropoietin receptor complexes sugg...Continue Reading
References
Feb 1, 1992·Molecular and Cellular Biology·A Yoshimura, H F Lodish
Jun 1, 1992·Trends in Biochemical Sciences·S L Pelech, J S Sanghera
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A L MuiG Krystal
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D M GormanA Miyajima
Oct 15, 1990·The Biochemical Journal·D Linnekin, W L Farrar
Dec 1, 1991·International Immunology·J SchreursA Miyajima
Jan 11, 1991·Methods in Enzymology·J E Ferrell, G S Martin
Jan 19, 1990·Science·N ItohA Miyajima
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R J IsfortJ N Ihle
Mar 28, 1986·Biochemical and Biophysical Research Communications·W S May, J N Ihle
May 16, 1985·Nature·W L FarrarW B Anderson
Mar 1, 1994·Baillière's Clinical Haematology·J N IhleF W Quelle
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·T ItoW S May
Mar 10, 1995·Cell·U KlingmüllerH F Lodish
Dec 1, 1993·Molecular and Cellular Biology·T YiJ N Ihle
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·E LiboiB Mathey-Prevot
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·O SilvennoinenJ N Ihle
Sep 15, 1993·European Journal of Biochemistry·P MayeuxS Gisselbrecht
Jun 1, 1993·European Journal of Biochemistry·C M HaysteadT A Haystead
Citations
Nov 22, 1996·The Journal of Biological Chemistry·L LiuG Krystal
Aug 5, 2000·The Journal of Biological Chemistry·Y MiyakawaK Kaushansky
Jan 14, 1999·Endocrinology·R HertanD M Robertson