Identification and Characterization of Anaplasma phagocytophilum Proteins Involved in Infection of the Tick Vector, Ixodes scapularis

PloS One
Margarita VillarJosé de la Fuente

Abstract

Anaplasma phagocytophilum is an emerging zoonotic pathogen transmitted by Ixodes scapularis that causes human granulocytic anaplasmosis. Here, a high throughput quantitative proteomics approach was used to characterize A. phagocytophilum proteome during rickettsial multiplication and identify proteins involved in infection of the tick vector, I. scapularis. The first step in this research was focused on tick cells infected with A. phagocytophilum and sampled at two time points containing 10-15% and 65-71% infected cells, respectively to identify key bacterial proteins over-represented in high percentage infected cells. The second step was focused on adult female tick guts and salivary glands infected with A. phagocytophilum to compare in vitro results with those occurring during bacterial infection in vivo. The results showed differences in the proteome of A. phagocytophilum in infected ticks with higher impact on protein synthesis and processing than on bacterial replication in tick salivary glands. These results correlated well with the developmental cycle of A. phagocytophilum, in which cells convert from an intracellular reticulated, replicative form to the nondividing infectious dense-core form. The analysis of A. phagocyt...Continue Reading

References

Jul 16, 1999·Journal of Clinical Microbiology·U G MunderlohJ L Goodman
Apr 4, 2000·Current Opinion in Microbiology·T Nogueira, M Springer
Aug 15, 2001·Antimicrobial Agents and Chemotherapy·P KraiczyK P Hunfeld
Dec 26, 2001·The Journal of Infectious Diseases·S D JauronU G Munderloh
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 23, 2003·Experimental & Applied Acarology·Katherine M KocanJose Carlos Garcia-Garcia
Mar 24, 2004·Veterinary Microbiology·Jose C Garcia-GarciaKatherine M Kocan
Sep 27, 2005·Journal of Structural Biology·David N Mastronarde
Feb 3, 2006·Expert Review of Vaccines·Kelly A BraytonWendy C Brown
Feb 17, 2006·PLoS Genetics·Julie C Dunning HotoppHervé Tettelin
May 24, 2006·The Journal of Experimental Medicine·Bindu SukumaranErol Fikrig
Dec 14, 2006·Molecular Microbiology·Amanda J McBroom, Meta J Kuehn
Oct 9, 2007·Methods : a Companion to Methods in Enzymology·Gabriele Multhoff
Feb 28, 2008·Molecular & Cellular Proteomics : MCP·Salvador Martínez-BartoloméJesús Vázquez
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Katherine M KocanEdmour F Blouin
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Jul 29, 2009·Comparative and Functional Genomics·Zorica ZivkovicJosé de la Fuente
Aug 29, 2009·Journal of Proteome Research·Pedro Navarro, Jesús Vázquez
Oct 13, 2009·Veterinary Parasitology·José de la FuenteAtilio J Mangold
Mar 4, 2010·Microbiology and Molecular Biology Reviews : MMBR·Terri N Ellis, Meta J Kuehn
Dec 15, 2010·Veterinary Microbiology·Yusuke MuraseKazuhiko Ohashi
Jul 8, 2011·Clinical Microbiology Reviews·Yasuko Rikihisa
Oct 20, 2011·Biochimica Et Biophysica Acta·Jill L Johnson

❮ Previous
Next ❯

Citations

Jan 1, 2016·Trends in Microbiology·José de la FuenteKatherine M Kocan
Apr 26, 2017·Frontiers in Cellular and Infection Microbiology·José de la FuenteRyan O M Rego
Jan 26, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mara BattilaniFrancesco Dondi
Aug 2, 2018·Expert Review of Proteomics·Sara Artigas-JerónimoMargarita Villar
May 21, 2016·Parasite Immunology·J de la FuenteC Maritz-Olivier
Jul 21, 2017·Frontiers in Cellular and Infection Microbiology·Marinela ContrerasJosé de la Fuente
Aug 12, 2020·Scientific Reports·Kateryna KotsarenkoLibor Grubhoffer
Jan 19, 2017·Expert Review of Proteomics·Margarita VillarJosé de la Fuente
Apr 2, 2021·Pathogens and Disease·Anya J O'NealJoao H F Pedra
Feb 4, 2019·Trends in Parasitology·Alejandro Cabezas-CruzJosé de la Fuente
Jul 1, 2021·Pathogens and Global Health·Cristiano SalataLesley Bell-Sakyi

❮ Previous
Next ❯

Methods Mentioned

BETA
protein folding
Flow cytometry
immunoprecipiation
immunoprecipitation
electron microscopy
electron tomography
PCR
Protein Assay
reverse phase chromatography
Protein Digestion

Software Mentioned

SerialEM
S4TE
STRING
genNorm
IMOD
Proteome Discoverer
STRAP for Researching Annotations
CellQuest Pro

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.