Identification and Characterization of c-di-GMP Metabolic Enzymes of Leptospira interrogans and c-di-GMP Fluctuations After Thermal Shift and Infection

Frontiers in Microbiology
Guohui XiaoXu'ai Lin

Abstract

Leptospirosis is a widespread zoonotic disease caused by pathogenic Leptospira species. The most common species, Leptospira interrogans, can transfer from contaminated soil or water to the human body. It is able to survive these changing environments through sensing and responding to the changes of environmental cues. Cyclic di-GMP (c-di-GMP) is a special secondary messenger in bacteria, which can respond to the environment and regulate diverse bacterial behaviors. The c-di-GMP levels in bacterial cells are regulated by diguanylatecyclases (DGC) and phosphodiesterases (PDE), which are responsible for synthesizing or hydrolyzing c-di-GMP, respectively. In this study, distribution and phylogenetics of c-di-GMP metabolic genes among 15 leptospiral species were systematically analyzed. Bioinformatics analysis revealed that leptospiral species contain a multitude of c-di-GMP metabolic genes. C-di-GMP metabolic genes in L. interrogans strain Lai 56601 were further analyzed and the results showed that these genes have very diverse expression patterns. Most of the putative DGCs and PDEs possess enzymatic activities, as determined by riboswitch-based dual-fluorescence reporters in vivo or HPLC in vitro. Furtherer analysis of subdomains ...Continue Reading

References

Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·B L Taylor, I B Zhulin
Dec 19, 2000·Infection and Immunity·J E NallyB Stevenson
Apr 9, 2001·Clinical Microbiology Reviews·P N Levett
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 4, 2003·The Lancet Infectious Diseases·Ajay R BhartiUNKNOWN Peru-United States Leptospirosis Consortium
Apr 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert P RyanJ Maxwell Dow
Aug 23, 2006·The Journal of Biological Chemistry·Beat ChristenUrs Jenal
Sep 14, 2006·The Journal of Biological Chemistry·Marina TarutinaMark Gomelsky
Feb 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·David K R KaraolisFrançois Malouin
Jun 30, 2007·Protein Science : a Publication of the Protein Society·Eduardo Henrique Silva SousaMarie-Alda Gilles-Gonzalez
Sep 22, 2009·Biochemistry·Jason R TuckermanMarie-Alda Gilles-Gonzalez
Dec 23, 2009·Microbes and Infection·Tatiane R OliveiraAna L T O Nascimento
Apr 14, 2010·Journal of Microbiological Methods·Christian SpanglerVolkhard Kaever
Aug 28, 2010·Bioorganic & Medicinal Chemistry·Shi Min ChingYulin Lam
Sep 29, 2011·Nature·Dara L BurdetteRussell E Vance
Jun 21, 2012·Molecular Plant-microbe Interactions : MPMI·Fenghuan YangChenyang He
Aug 2, 2012·Antimicrobial Agents and Chemotherapy·Karthik SambanthamoorthyChristopher M Waters
Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Ute RömlingMark Gomelsky
Feb 17, 2015·Environmental Microbiology·Loni Townsley, Fitnat H Yildiz
Jun 10, 2015·Journal of Bacteriology·Shan-Ho Chou, Michael Y Galperin

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Methods Mentioned

BETA
PCR
protein assay
High Performance Liquid Chromatography
thermal shift
three hybrid
genetic modification

Software Mentioned

blastP
EvolView
PhyML
Spectramax

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