Identification and characterization of cAMP-dependent protein kinase and its possible direct interactions with protein phosphatase-1 in marine dinoflagellates

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
J F DawsonC F Holmes

Abstract

We have examined the nature of signal transduction involving reversible protein phosphorylation in marine Prorocentrale species. Of particular interest is the marine dinoflagellate Prorocentrum lima in which the tumour promoter okadaic acid is produced and may interfere with signal transduction. We have identified cAMP-dependent protein kinase (PKA) activity in P. lima, P. micans, and P. minimum. The P. lima enzyme was characterized biochemically and appears to consist of two different isoforms in the R2C2 configuration. Whole cell extracts of P. micans and P. minimum treated with the specific PKA inhibitor peptide PKI (5-24) or cAMP demonstrated altered intensities of phosphopeptide 32P labeling, most likely involving regulation of a protein phosphatase via PKA activity. A primary candidate for PKA regulation is protein phosphatase-1 (PP-1), which in P. lima possesses a classical PKA consensus phosphorylation site. We demonstrate that a peptide fragment of PP-1 from P. lima corresponding to this PKA phosphorylation site can be effectively phosphorylated by PKA and dephosphorylated by calcineurin. We speculate that PP-1 activity among several lower eukaryotes may be mediated directly by reversible phosphorylation. Higher eukary...Continue Reading

References

Feb 1, 1976·The Journal of protozoology·A R Loeblich
Nov 1, 1992·Plant Molecular Biology·S J Rundle, J B Nasrallah
Apr 1, 1991·Current Opinion in Cell Biology·G S McKnight
Dec 1, 1991·Molecular and Biochemical Parasitology·N E Erondu, J E Donelson
Jan 1, 1990·Annual Review of Biochemistry·S S TaylorW Yonemoto
Dec 27, 1990·European Journal of Biochemistry·V DombrádiP T Cohen
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R MutzelM Veron
Jan 1, 1993·Plant Molecular Biology·R D Smith, J C Walker
Nov 1, 1993·Molecular and Cellular Biochemistry·J L MeinkothJ R Feramisco
Dec 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D A Walsh, S M Van Patten
Jan 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·S A Norman, D M Mott
Sep 13, 1993·Biochimica Et Biophysica Acta·S O DøskelandB T Gjertsen
Oct 1, 1991·Plant Physiology·R D Smith, J C Walker

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Citations

Jul 11, 2001·Journal of Experimental Marine Biology and Ecology·T A. Leighfield, F M. Van Dolah
Nov 15, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Tod A LeighfieldFrances M Van Dolah
Feb 11, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J F Dawson, C F Holmes

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