Identification and characterization of chitin deacetylase2 from the American white moth, Hyphantria cunea (Drury)

Gene
Xiaoping YanXiaoyun Wang

Abstract

Chitin deacetylases (CDAs) are enzymes that catalyze the conversion of chitin into chitosan, thereby influence the mechanical and permeability properties of structures such as the cuticle and peritrophic matrices. The full length cDNAs of chitin deacetylase2 (CDA2) genes from Hyphantria cunea were fully cloned by PCR amplification. Two cDNA sequences of HcCDA2 were searched from transcriptome of H. cunea and named as HcCDA2a and HcCDA2b. The deduced protein sequences showed that HaCDA2a and HaCDA2b are synthesized as preproteins of 524 and 518 amino acid residues with an 18-amino acid signal peptide, respectively. HcCDA2a and HcCDA2b contained a chitin-binding domain (ChBD), a low-density lipoprotein receptor class A domain (LDLa) and a polysaccharide deacetylase-like catalytic domain (CDA). Gene expression analyses results showed that HcCDA2a and HcCDA2b were both expressed at the head, integument, foregut, midgut, hindgut, Malpighian tubules and fat body, as well as the 1st to 5th days of fifth instar larvae. Western blot analyses revealed that HcCDA2 protein was highly abundant in the head and integument, and the developmental expression result in the fifth instars showed that HcCDA2 was highly present at the first two days....Continue Reading

References

Jan 1, 1993·Archives of Insect Biochemistry and Physiology·E Cohen
Mar 21, 1998·Insect Biochemistry and Molecular Biology·K J Kramer, S Muthukrishnan
Jun 17, 2000·Trends in Biotechnology·I TsigosV Bouriotis
Nov 12, 2003·The Journal of Experimental Biology·Hans Merzendorfer, Lars Zimoch
Mar 5, 2005·Journal of Morphology·Bernard MoussianChristiane Nüsslein-Volhard
Aug 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yasuyuki ArakaneKarl J Kramer
Mar 10, 2009·Insect Biochemistry and Molecular Biology·Yasuyuki ArakaneRichard W Beeman
Oct 10, 2009·Cellular and Molecular Life Sciences : CMLS·Yasuyuki Arakane, Subbaratnam Muthukrishnan
Jan 30, 2014·International Journal of Molecular Sciences·Xiao-Wu ZhongPing Zhao

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Citations

Mar 1, 2020·Archives of Insect Biochemistry and Physiology·Yan LiuDong Fan
Apr 5, 2020·International Journal of Molecular Sciences·Wen-Jia YangDao-Chao Jin
Dec 22, 2019·International Journal of Molecular Sciences·Hai-Zhong YuZhan-Jun Lu

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