Identification and characterization of circRNAs in Pyrus betulifolia Bunge under drought stress

PloS One
Jinxing WangYouhong Chang

Abstract

Circular RNAs (circRNAs) play important roles in miRNA function and transcriptional control. However, little is known regarding circRNAs in the pear. In this study, we identified circRNAs using deep sequencing and analyzed their expression under drought stress. We identified 899 circRNAs in total, among which 33 (23 upregulated, 10 downregulated) were shown to be dehydration-responsive. We performed GO and KEGG enrichment analysis to predict the functions of differentially expressed circRNAs. 309 circRNAs were predicted to act as sponges for 180 miRNAs. A circRNA-miRNA co-expression network was constructed based on correlation analysis between the differentially expressed circRNAs and their miRNA binding sites. Our study will provide a rich genetic resource for the discovery of genes related to drought stress, and can readily be applied to other fruit trees.

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Citations

Jun 9, 2020·Frontiers in Molecular Biosciences·Peijing ZhangMing Chen
Jan 31, 2020·Frontiers in Molecular Biosciences·Ashirbad GuriaGopal Pandi
Oct 17, 2019·Animals : an Open Access Journal From MDPI·Yongfu LaMingxing Chu
May 21, 2021·Functional & Integrative Genomics·Sailaja BhogireddyHimabindu Kudapa

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Datasets Mentioned

BETA
SRP
150567

Methods Mentioned

BETA
PCR
RNA-seq

Software Mentioned

DESeq2
circ
cBot Cluster Generation System
GOseq
CIRI
psRobot
CircRNA
find
KOBAS
Primer5

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