Identification and characterization of coenzyme B12-dependent glycerol dehydratase- and diol dehydratase-encoding genes from metagenomic DNA libraries derived from enrichment cultures.

Applied and Environmental Microbiology
Anja KnietschR Daniel

Abstract

To isolate genes encoding coenzyme B(12)-dependent glycerol and diol dehydratases, metagenomic libraries from three different environmental samples were constructed after allowing growth of the dehydratase-containing microorganisms present for 48 h with glycerol under anaerobic conditions. The libraries were searched for the targeted genes by an activity screen, which was based on complementation of a constructed dehydratase-negative Escherichia coli strain. In this way, two positive E. coli clones out of 560,000 tested clones were obtained. In addition, screening was performed by colony hybridization with dehydratase-specific DNA fragments as probes. The screening of 158,000 E. coli clones by this method yielded five positive clones. Two of the plasmids (pAK6 and pAK8) recovered from the seven positive clones contained genes identical to those encoding the glycerol dehydratase of Citrobacter freundii and were not studied further. The remaining five plasmids (pAK2 to -5 and pAK7) contained two complete and three incomplete dehydratase-encoding gene regions, which were similar to the corresponding regions of enteric bacteria. Three (pAK2, -3, and -7) coded for glycerol dehydratases and two (pAK4 and -5) coded for diol dehydratas...Continue Reading

References

Dec 1, 1979·Canadian Journal of Microbiology·J N Saddler, A W Khan
Mar 22, 1976·Biochemical and Biophysical Research Communications·T TorayaS Fukui
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jan 28, 1999·The Journal of Biological Chemistry·T Toraya, K Mori
May 29, 1999·Applied and Environmental Microbiology·M T CottrellD L Kirchman
Jan 11, 2001·Biochimica Et Biophysica Acta·G ChotaniK Sanford
May 15, 2002·FEMS Microbiology Letters·Nicolas SauvageotJean-Marie Laplace
Sep 17, 2002·European Journal of Biochemistry·Mamoru YamanishiTetsuo Toraya
Dec 17, 2002·The Journal of General and Applied Microbiology·Frank HosterGerhard Gottschalk
Apr 1, 1992·Applied and Environmental Microbiology·B DabrockG Gottschalk

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Citations

Sep 15, 2004·Applied Microbiology and Biotechnology·Seon-Woo LeeKwang Yun Cho
Feb 1, 2005·Applied Microbiology and Biotechnology·Y DoleyresC Lacroix
Mar 31, 2007·Applied Microbiology and Biotechnology·Christel SchmeisserWolfgang R Streit
Sep 18, 2009·Applied Microbiology and Biotechnology·Carola Simon, Rolf Daniel
Feb 5, 2013·Applied Microbiology and Biotechnology·Satish Kumar AinalaSunghoon Park
Dec 19, 2007·Extremophiles : Life Under Extreme Conditions·Meixiang XuFengping Wang
Apr 10, 2010·Journal of Industrial Microbiology & Biotechnology·Yu-Tze HorngPo-Chi Soo
Jun 1, 2008·Indian Journal of Microbiology·J VakhluB N Johri
Oct 29, 2003·Current Opinion in Biotechnology·Charles E Nakamura, Gregory M Whited
Jun 3, 2005·Nature Reviews. Microbiology·Rolf Daniel
Jun 3, 2005·Nature Reviews. Microbiology·Patrick Lorenz, Jürgen Eck
Dec 11, 2007·Applied and Environmental Microbiology·Eu Jin ChungSeon-Woo Lee
Dec 21, 2010·Applied and Environmental Microbiology·Carola Simon, Rolf Daniel
Dec 8, 2005·Applied and Environmental Microbiology·He Kyoung LimSeon-Woo Lee
Dec 8, 2005·Applied and Environmental Microbiology·Matthew T CottrellDavid L Kirchman
Jun 1, 2013·Genome Announcements·Satish Kumar AinalaSunghoon Park
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Jo Handelsman
Jun 12, 2008·Annual Review of Microbiology·Ainslie E F LittleJo Handelsman
Jul 10, 2007·Journal of Nutritional Science and Vitaminology·Takahiro SakaiTetsuo Toraya
Mar 9, 2007·Microbial Ecology·George A KowalchukJohannes A van Veen
Sep 28, 2015·Biotechnology Advances·Mamoon Rashid, Ulrich Stingl
Mar 6, 2010·Trends in Microbiology·Yin Chen, J Colin Murrell
Apr 14, 2009·Journal of Microbiological Methods·C L RandazzoE Neviani
Sep 9, 2008·Biotechnology Advances·Gervásio Paulo da SilvaJonas Contiero
Mar 12, 2016·Biotechnology for Biofuels·Wei JiangBaishan Fang
Mar 17, 2009·Biotechnology Journal·Jagtar SinghNavneet Batra
Aug 30, 2005·Trends in Biotechnology·Teca Calcagno GalvãoVíctor de Lorenzo
Dec 1, 2004·Annual Review of Genetics·Christian S RiesenfeldJo Handelsman
Aug 6, 2013·FEMS Microbiology Reviews·Eva BoonRobert G Beiko
Aug 7, 2004·Current Opinion in Biotechnology·Wolfgang R StreitKarl-Erich Jaeger
Jan 18, 2017·Journal of Industrial Microbiology & Biotechnology·Mugesh SankaranarayananSunghoon Park
Jun 15, 2004·Current Opinion in Biotechnology·Rolf Daniel
Aug 10, 2006·Biotechnology Journal·Martin LangerPatrick Lorenz

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